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The chick embryo nodose ganglion: Effects of nerve growth factor in culture

  • Published:
Journal of Neurocytology

Summary

Nodose ganglia from 8-day-old chick embryos were cultured in collagen gels for 2 days, with or without added nerve growth factor (NGF), in order to discover whether the nodose neurons, derived from an epidermal placode, are susceptible to this trophic factor. Neuronal survival and neurite outgrowth were stimulated only after addition of NGF, and the enhancing effects could be blocked by introducing antibodies to NGF. Stereological analysis of ganglia sectioned for light microscopy showed that the NGF-treated neurons increased their volume by about 50%, as did the nodose neurons in ovo from the eighth to the tenth day of incubation. The volume density, however, was significantly lowerin vitro indicating a limited cell death during culture despite the presence of exogenously supplied NGF. The number of neurofilaments and microtubules increased in the cell centre of neurons treated with NGF; this region also showed numerous dense bodies and an extensive Golgi complex by electron microscopy. Ultrastructural similarities between neurons responding to NGF and neurons undergoing the axon reaction which follows axotomy are indicated. A role for a trophic factor resembling NGF in the normal development of placode-derived neurons of the sensory cranial ganglia is suggested.

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References

  • Barde, Y.-A., Edgar, D. &Thoenen, H. (1979) The effect of nerve growth factor and glial conditioned medium on the survival in culture of sensory neurones of different embryonic ages.Neurosdence Letters Suppl.3, S332.

    Google Scholar 

  • Campenhout, E. Van (1937) Le rôle des placodes épiblastiques au cours du développement embryonnaire du porc et du poulet.Bulletin de L'Académie Royale de Médecine de Belgique 2, 169–84.

    Google Scholar 

  • Duchen, L. W. &Scaravilli, F. (1977) Quantitative and electron microscopic studies of sensory ganglion cells of the Sprawling mouse.Journal of Neurocytology 6, 465–81.

    Google Scholar 

  • Ebendal, T. (1979) Stage-dependent stimulation of neunte outgrowth exerted by nerve growth factor and chick heart in cultured embryonic ganglia.Developmental Biology 72, 276–90.

    Google Scholar 

  • Ebendal, T., Belew, M., Jacobson, C.-O. &Porath, J. (1979) Neurite outgrowth elicited by embryonic chick heart: Partial purification of the active factor.Neurosdence Letters 14, 91–5.

    Google Scholar 

  • Ebendal, T. &Hedlund, K.-O. (1974) Histology of the chick embryo trigeminal ganglion and initial effects of its cultivation with and without nerve growth factor.Zoon 2, 25–35.

    Google Scholar 

  • Ebendal, T. &Hedlund, K.-O. (1975) Effects of nerve growth factor on the chick embryo trigeminal ganglion in culture.Zoon 3, 33–47.

    Google Scholar 

  • Ebendal, T. &Jacobson, C.-O. (1977) Tests of possible role of NGF in neurite outgrowth stimulation exerted by glial cells and heart expiants in culture.Brain Research 131, 373–8.

    Google Scholar 

  • Ebendal, T., Jordell-Kylberg, A. &Söderström, S. (1978) Stimulation by tissue explants on nerve fibre outgrowth in culture. InFormshaping Movements in Neurogenesis (edited byJacobson, C.-O. andEbendal, T.), pp. 235–243. Stockholm: Almqvist & Wiksell International.

    Google Scholar 

  • Fenton, E. L. (1970) Tissue culture assay of nerve growth factor and of the specific antiserum.Experimental Cell Research 59, 383–92.

    Google Scholar 

  • Hamburger, V. (1961) Experimental analysis of the dual origin of the trigeminal ganglion in the chick embryo.Journal of Experimental Zoology 148, 91–124.

    Google Scholar 

  • Hamburger, V. (1962) Specificity in neurogenesis.Journal of Cellular and Comparative Physiology 60 (Suppl. 1), 81–92.

    Google Scholar 

  • Hamburger, V. &Hamilton, H. (1951) A series of normal stages in the development of the chick embryo.Journal of Morphology 88, 49–92.

    Google Scholar 

  • Hedlund, K.-O. &Ebendal, T. (1978) Different ganglia from the chick embryo in studies on neuron development in culture. InFormshaping Movements in Neurogenesis (edited byJacobson, C.-O. andEbendal, T.), pp. 217–23. Stockholm: Almqvist & Wiksell International.

    Google Scholar 

  • Helfand, S. L., Riopelle, R. J. &Wessells, N. K. (1978) Non-equivalence of conditioned medium and nerve growth factor for sympathetic, parasympathetic and sensory neurons.Experimental Cell Research 113, 39–45.

    Google Scholar 

  • Hinek, A., Thyberg, J. &Friberg, U. (1977) Electron microscopic studies on embryonic chick spinal ganglia cells: relationship between microtubules and the Golgi complex.Journal of Neurocytology 6, 13–25.

    Google Scholar 

  • Levi-montalcini, R. (1962) Analysis of a specific nerve growth factor and of its antiserum.Scientific Reports from Istituto Superiore di Sanità 2, 345–68.

    Google Scholar 

  • Levi-Montalcini, R. &Angeletti, P. U. (1968) Nerve growth factor.Physiological Reviews 48, 534–69.

    Google Scholar 

  • Levi-Montalcini, R., Caramia, F., Luse, S. A. &Angeletti, P. U. (1968)In vitro effects of nerve growth factor on the fine structure of the sensory nerve cells.Brain Research 8, 347–62.

    Google Scholar 

  • Lieberman, A. R. (1971) The axon reaction: A review of the principal features of perikaryal responses to axon injury.International Review of Neurobiology 14, 49–124.

    Google Scholar 

  • Lindsay, R. M. (1979) Adult rat brain astrocytes support survival of both NGF-dependent and NGF-insensitive neurons.Nature 282, 80–2.

    Google Scholar 

  • Lindsay, R. M. &Tarbit, J. (1979) Developmentally regulated induction of neurite outgrowth from immature chick sensory neurons (DRG) by homogenates of avian or mammalian heart, liver and brain.Neuroscience Letters 12, 195–200.

    Google Scholar 

  • Mobley, W. C., Server, A. C., Ishii, D. N., Riopelle, R. J. &Shooter, E. M. (1977a) Nerve growth factor (Part one).New England Journal of Medicine 297, 1096–104.

    Google Scholar 

  • Mobley, W. C., Server, A. C., Ishii, D. N., Riopelle, R. J. &Shooter, E. M. (1977b) Nerve growth factor (Part two).New England Journal of Medicine 297, 1149–58.

    Google Scholar 

  • Mobley, W. C., Server, A. C., Ishii, D. N., Riopelle, R. J. &Shooter, E. M. (1977c) Nerve growth factor (Part three).New England Journal of Medicine 297, 1211–8.

    Google Scholar 

  • Narayanan, C. H. &Narayanan, Y. (1978) Placodal and neural crest contributions to the glossopharyngeo-vagal complex studied by the method of interspecific transplantations between quail and chick embryos.Society for Neuroscience Abstracts 4, 122.

    Google Scholar 

  • Moden, D. (1978) The control of avian cephalic neural crest cytodifferentiation. II. Neural tissues.Developmental Biology 67, 313–29.

    Google Scholar 

  • Novikoff, P. M., Novikoff, A. B., Quintana, N. &Hauw, J.-J. (1971) Golgi apparatus, GERL and lysosomes of neurons in rat dorsal root ganglia, thin section cytochemistry.Journal of Cell Biology 50, 859–86.

    Google Scholar 

  • Olson, L., Ebendal, T. &Seiger, A. (1979) NGF and anti-NGF: Evidence against effects on fiber growth in locus coeruleus from cultures of perinatal CNS tissues.Developmental Neurosdence 2, 160–76.

    Google Scholar 

  • Pannese, E. (1974) The histogenesis of the spinal ganglia.Advances in Anatomy, Embryology and Cell Biology 47, 1–97.

    Google Scholar 

  • Pannese, E. (1976) An electron microscopic study of cell degeneration in chick embryo spinal ganglia.Neuropathology and Applied Neurobiology 2, 247–67.

    Google Scholar 

  • Romanoff, A. L. (1960)The Avian Embryo. New York: Macmillan.

    Google Scholar 

  • Terni, T. (1924) II ganglio toracico e la porzione cervicale del vago negli Uccelli.Archivio Italiano di Anatomia e di Embriologia 21, 404–34.

    Google Scholar 

  • Varon, S. &Bunge, R. P. (1978) Trophic mechanisms in the peripheral nervous system.Annual Reviews of Neurosdence 1, 327–61.

    Google Scholar 

  • Varon, S., Nomura, J. &Shooter, E. M. (1967) The isolation of the mouse nerve growth factor protein in a high molecular weight form.Biochemistry 6, 2202–9.

    Google Scholar 

  • Weibel, E. R. (1969) Stereological principles for morphometry in electron microscopic cytology.International Review of Cytology 26, 235–302.

    Google Scholar 

  • Weis, P. (1970) Thein vitro effect of the nerve growth factor on chick embryo spinal ganglia — a light-microscopic evaluation.Journal of Embryology and Experimental Morphology 24, 381–92.

    Google Scholar 

  • Zelená, J. (1971) Neurofilaments and microtubules in sensory neurons after peripheral nerve section.Zeitschrift für Zellforschung und mikroskopische Anatomie 117, 191–211.

    Google Scholar 

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Authors and Affiliations

  1. Department of Zoology, Uppsala University, Box 561, S-751 22, Uppsala, Sweden

    Kjell-Olof Hedlund & Ted Ebendal

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  1. Kjell-Olof Hedlund
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  2. Ted Ebendal
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Hedlund, KO., Ebendal, T. The chick embryo nodose ganglion: Effects of nerve growth factor in culture. J Neurocytol 9, 665–682 (1980). https://doi.org/10.1007/BF01205032

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  • DOI: https://doi.org/10.1007/BF01205032

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