Abstract
An extracellular polygalacturonase (EC 3.2.1.15) fromGeotrichum candidum ATCC 34614 grown onsauerkraut brine was produced and characterized. Polygalacturonic acid markedly increased the enzyme yield in the brine. The fungus produced the highest activity (290 U/l) in brine with 0.3% (w/v) polygalacturonic acid. The pH and temperature optima of the enzymes were 4.5 to 5.0 and 30°C, respectively. It was stable from pH 4.0 to 5.8 and at 30°C but lost its activity at higher temperatures. The Km and Vmax values for polygalacturonic acid were 4.2 mg/ml and 0.19mm galacturonic acid/min, respectively. The enzyme was not substrate inhibited.
Similar content being viewed by others
References
Barash, I. 1980 Liberation of polygalacturonase during spore germination byGeotrichum candidum. InCompendium of Soil Fungi, eds Domsch, K.H., Gams, W. & Anderson, T. pp. 1364–1371, New York: Academic Press.
Boyce, C.O.L. 1986Novós Handbook of Practical Biotechnology. Bagsvaerd, Denmark: NovoIndustri A/S.
Collmer, A., Ried, J.L. & Mount, M.S. 1988 Pectic enzyme assays.Methods in Enzymology 161, 329–335.
Davis, L.L. & Baudoin, A.B. 1986 Effect of osmotic potential onGeotrichum candidum: growth, polygalacturonase production and polygalacturonase action.Physiological and Molecular Plant Pathology 28, 53–60.
Fogarty, W.M. & Kelly, C.T. 1983 Pectic enzymes. InMicrobial Enzymes and Biotechnology, ed Fogarty, W.M. pp. 131–182, New York: Applied Sciences Publishers.
Gomez-Ruiz, L., Garcia-Garibay, M. & Barzana, E. 1988 Utilization of endo-polygalacturonase fromKluyveromyces fragilis in the clarification of apple juice.Journal of Food Science 53, 1236–1237.
Hang, Y.D., Splittstoesser, D.F. & Landschoot, R.E. 1974 Propagation ofGeotrichum candidum in acid brine.Applied Microbiology 27, 807–808.
Jay, J.M. 1986Modern Food Microbiology, 3rd edn. New York: Van Nostrand Company.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Hang, Y.D., Woodams, E.E. Production and characterization of polygalacturonase fromGeotrichum candidum . World J Microbiol Biotechnol 8, 480–482 (1992). https://doi.org/10.1007/BF01201944
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01201944