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Major organ-specific glycoproteins in isolated brain and kidney membranes identified as Na,K-ATPase subunits by combined glycan-, lectin-, and immunoblotting

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Abstract

In the present work combined glycan-, lectin-, and immunoblotting of isolated brain and kidney membranes shows that the α and β subunits of Na,K-ATPase are the most abundant glycoproteins. Further,Datura stramonium andGalanthus nivalis agglutinins recognize the Na,K-ATPase subunits in a mutually exclusive manner in membranes from human, rabbit and rat brain or human, rabbit, rat, pig and dog kidney indicating the presence of species-independent organ-typical glycoforms. The glycosylation status is not related to the ouabain-sensitivity. Taken together, the data reveals organ-specific glycoforms of Na,K-ATPase which might have roles for organ identification and recognition.

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Abbreviations

NKA:

Na,K-ATPase (EC 3.6.1.37)

PAGE:

polyacrylamide gel electrophoresis in dodecylsulfate

Con-A:

Concanavalin A

DSA:

Datura stramonium agglutinin

GNA:

Galanthus nivalis agglutinin

MAA:

Maackia amurensis agglutinin

PNA:

Peanut agglutinin

SNA:

Sambucus nigra agglutinin

WGA:

Wheat germ agglutinin

K:

kidney

B:

brain

Cr:

Crude

De:

Detergent-treated

Fe:

fetuin

Ct:

creatinase

I-blot:

immuno-blot

L-blot:

lectin-blot

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Authors and Affiliations

  1. The Laboratory of Experimental Cell Therapeutics, Geneva University Medical School, CH-1211, Geneva 4, Switzerland

    Mounja Benallal & Beatrice M. Anner

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  1. Mounja Benallal
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  2. Beatrice M. Anner
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Benallal, M., Anner, B.M. Major organ-specific glycoproteins in isolated brain and kidney membranes identified as Na,K-ATPase subunits by combined glycan-, lectin-, and immunoblotting. Biosci Rep 15, 21–36 (1995). https://doi.org/10.1007/BF01200212

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