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Indirect spectrophotometric determination of reducing sugars and ascorbic acid using phenolphthalin as reagent

Indirekte spektrophotometrische Bestimmung reduzierender Zucker und der Askorbinsäure mit Phenolphthalin

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Summary

An indirect spectrophotometric method has been developed for the determination of reducing sugars and ascorbic acid. A known excess of ferricyanide is allowed to react with the reductant, and the surplus ferricyanide is determined spectrophotometrically with phenolphthalin. The method is simple and reproducible, with an error of less than 2%.

Zusammenfassung

Ein gemessener Überschuß von Eisen(III) cyanid wird mit der reduzierenden Probe umgesetzt und der nicht verbrauchte Anteil mit Phenol-phthalin spektrophotometrisch bestimmt. Das Verfahren ist einfach und gut reproduzierbar. Der Fehler ist geringer als 2%.

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References

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Authors and Affiliations

  1. Chemical Research Laboratory, Faculty of Engineering, Ain Shams University, Abbassia, Cairo, Egypt

    Salah Shahine & Rafaa Mahmoud

Authors
  1. Salah Shahine
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  2. Rafaa Mahmoud
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Shahine, S., Mahmoud, R. Indirect spectrophotometric determination of reducing sugars and ascorbic acid using phenolphthalin as reagent. Mikrochim Acta 73, 119–122 (1980). https://doi.org/10.1007/BF01197239

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  • DOI: https://doi.org/10.1007/BF01197239

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