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Use of electroenzyme catalysis in organic and biochemical analysis

Über die Verwendung der elektro-enzymatischen Katalyse in der organischen und biochemischen Analyse

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Summary

A method is proposed for the electroenzymic determination of alcohols. The biocatalyst used in the potentiometric sensor is an enzyme-coenzyme system (alcohol dehydrogenase and nicotinamide adenine dinucleotide) immobilized on oxidized spectral grade graphite. A mathematical model for the response signals is developed.

Zusammenfassung

Ein Verfahren zur elektro-enzymatischen Bestimmung von Alkoholen wurde vorgeschlagen. Der in dem potentiometrischen Sensor verwendete Biokatalysator ist ein auf oxydiertem Spektralgraphit immobilisiertes Enzym-Coenzym-System (Alkoholdehydrogenase und Nikotinamid-Adenin-dinukleotid). Ein mathematisches Modell des registrierten Signals wurde ausgearbeitet.

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Authors and Affiliations

  1. Kazakh State University, Alma-Ata, USSR

    P. P. Gladyshev, V. P. Gladyshev, V. Sh. Mamleev & S. V. Kovalyova

Authors
  1. P. P. Gladyshev
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  2. V. P. Gladyshev
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  3. V. Sh. Mamleev
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  4. S. V. Kovalyova
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Additional information

NAD+ and NADH are the generally accepted notations for the oxidized and reduced forms of NAD, respectively.

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Gladyshev, P.P., Gladyshev, V.P., Mamleev, V.S. et al. Use of electroenzyme catalysis in organic and biochemical analysis. Mikrochim Acta 76, 289–299 (1981). https://doi.org/10.1007/BF01196745

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  • DOI: https://doi.org/10.1007/BF01196745

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