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A simplified procedure for the gas chromatographic determination of nicotine: Application of the method to mouse skin

Ein vereinfachtes Verfahren zur gas-chromatographischen Bestimmung von Nikotin. Anwendung auf die Haut von Mäusen

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Summary

The gas Chromatographic procedure can be used for the rapid and accurate determination of small amounts of nicotine in biological material. Application of the method proved successful in the determination of the disappearance of nicotine from mouse skin following the application of this alkaloid directly to the skin surface. A plot ofμg of nicotine per mg DNA or protein of mouse skin versus the time in hr following the application of nicotine showed that the biological half-life of nicotine in mouse skin over the first 6 hr is 1.2 hr.

Zusammenfassung

Das beschriebene Verfahren kann zur raschen und genauen Bestimmung geringer Mengen Nikotin in biologischem Material dienen. Das Verschwinden des Nikotins nach dessen Applikation auf die Hautoberfläche von Mäusen wurde damit untersucht. Trägt manμg Nikotin/mg DNS der Mäusehaut gegen die Zeit nach Applikation des Nikotins auf, so zeigt sich, daß die biologische Halbzeit des Nikotins in der Mäusehaut innerhalb der ersten 6 Stunden 1,2 Stunden beträgt.

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Authors and Affiliations

  1. Orchard Park Laboratories, Roswell Park Memorial Institute (NY State Department of Health), Buffalo

    C. Carruthers & A. Neilson

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  1. C. Carruthers
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  2. A. Neilson
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Carruthers, C., Neilson, A. A simplified procedure for the gas chromatographic determination of nicotine: Application of the method to mouse skin. Mikrochim Acta 74, 59–66 (1980). https://doi.org/10.1007/BF01196486

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  • DOI: https://doi.org/10.1007/BF01196486

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