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Investigation of the causes of the decrease in enzyme induction after prolonged administration of a hormone: Reception of cortisol in rat liver cells

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Conclusions

  1. 1.

    In animals that received cortisol for 20 days or more the distribution of [3H]cortisol introducedin vivo among the subcellular fractions of the liver was substantially changed in comparison with the control: The relative content of the labeled hormone in the cytosol was decreased, and that in the microsomes, nuclei, and chromatin was increased.

  2. 2.

    In experimentsin vivo the binding of [3H]cortisol by the receptor proteins of the liver cytosol of experimental rats was significantly lower than in the control with a simultaneous increase in the incorporation into the nuclei and chromatin.

  3. 3.

    Incorporation of [3H]cortisol into the chromatin in the case ofin vitro incubation of a reconstituted homogenate of nuclei and cytosol with the labeled hormone was the highest in the combination of nuclei of experimental rats with cytosol of intact rats. When the nuclei of experimental or intact rats were incubated with cytosol of experimental animals, the binding of [3H]cortisol in the chromatin was 30% lower than when the nuclei were incubated with the cytosol of intact rats.

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Literature cited

  1. G. Weber, R. L. Singhal, N. B. Stamm, et al., Fed. Proc,24, 745–754 (1965).

    Google Scholar 

  2. F. T. Kenney and F. J. Kull, Proc. Natl. Acad. Sci. USA,50, 493–495 (1963).

    Google Scholar 

  3. R. I. Salganik, S. V. Argutinskaya, N. P. Mertvetsov, et al., Mol. Biol.,2, 721–725 (1968).

    Google Scholar 

  4. N. P. Mertvetsov, V. A. Saprykin, S. V. Argutinskaya, et al., Probl. Éndokrinol., No. 5, 78–81 (1971).

    Google Scholar 

  5. R. I. Salganik, S. V. Argutinskaya, and R. I. Bersimbaev, Experientia,27, 53–54 (1971).

    Google Scholar 

  6. R. I. Salganik, N. A. Solov'eva, and V. F. Drevich, Dokl. Akad. Nauk SSSR,204, 489–491 (1973).

    Google Scholar 

  7. M. Beato, W. Braendle, D. Biesewig, et al., Biochim. Biophys. Acta,208, 125–136 (1970).

    Google Scholar 

  8. I. D. Baxter, G. G. Rousseau, M. C. Benson, et al., Proc. Natl. Acad. Sci. USA,69, 1892–1896 (1972).

    Google Scholar 

  9. T. M. Morozova and R. I. Salganik, Mol. Biol.,3, 745–747 (1969).

    Google Scholar 

  10. M. Beato, D. Biesewig, W. Braendle, et al., Biochim. Biophys. Acta,192, 494–507 (1969).

    Google Scholar 

  11. S. V. Argutinskaya, V. L. Knorre, L. Yu. Erimova, et al., Mol. Biol.,7, 802–808 (1973).

    Google Scholar 

  12. K. Barton, Biochem. J.,62, 315–323 (1956).

    Google Scholar 

  13. O. H. Lowry, N. J. Rosebrough, A. L. Farr, et al., J. Biol. Chem.,193, 265–275 (1951).

    Google Scholar 

  14. W. E. Groves, F. C. Davis, and B. H. Sells, Anal. Biochem.,22, 195 (1968).

    Google Scholar 

  15. V. A. Shul'ga, N. P. Mertvetsov, V. G. Selyatitskaya, et al., Dokl. Akad. Nauk SSSR,213, 452–454 (1973).

    Google Scholar 

  16. J. R. Shaskas and G. D. Bottoms, Proc. Soc. Exp. Biol. (N.Y.),147, 232–238 (1974).

    Google Scholar 

  17. E. Milgrom, M. Atger, and E. E. Baulieu, Biochemistry (Wash.),12, 5198–5205 (1973).

    Google Scholar 

  18. M. Beato, M. Kalimi, W. Beato, et al., Endocrinology,94, 377–387 (1973).

    Google Scholar 

  19. A. N. Panov and V. G. Shalyapina, Probl. Éndokrinol., No. 2, 75–77 (1968).

    Google Scholar 

  20. Ya. L. Germanyuk, in: News of Hormones and the Mechanism of their Action [in Russian], Kiev (1977), pp. 91–100.

  21. S. V. Argutinskaya and V. G. Selyatitskaya, in: Structure and Functions of the Cell Nucleus [in Russian], Novosibirsk (1975), pp. 118–119.

  22. K. Einarsson and J. A. Gustafsson, Eur. J. Biochem.,32, 197–206 (1973).

    Google Scholar 

  23. A. N. Conney, Pharmacol. Rev.,19, 317–366 (1967).

    Google Scholar 

  24. S. V. Nedel'kina, S. V. Argutinskaya, and R. I. Salganik, Vopr. Med. Khim., No. 1, 65–69 (1972).

    Google Scholar 

  25. A. Poland, E. Glover, and A. S. Kende, J. Biol. Chem.,251, 5936–4946 (1976).

    Google Scholar 

  26. T. M. Guenther and D. W. Nebert, J. Biol. Chem.,252, 8981–8989 (1977).

    Google Scholar 

  27. G. Litvach, R. Filler, and M. N. Cake, Vestn. Akad. Med. Nauk SSSR, No. 3, 42–46 (1977).

    Google Scholar 

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Authors and Affiliations

  1. Laboratory of Molecular Genetics, Institute of Cytology and Genetics, Siberian Branch, Academy of Sciences of the USSR, Novosibirsk

    S. V. Argutinskaya, T. V. Arshinova, V. G. Selyatitskaya & R. I. Salganik

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  1. S. V. Argutinskaya
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  2. T. V. Arshinova
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  3. V. G. Selyatitskaya
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  4. R. I. Salganik
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Additional information

Translated from Problemy Éndokrinologii, Vol. 27, No. 3, pp. 43–48, May–June, 1981.

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Argutinskaya, S.V., Arshinova, T.V., Selyatitskaya, V.G. et al. Investigation of the causes of the decrease in enzyme induction after prolonged administration of a hormone: Reception of cortisol in rat liver cells. Neurosci Behav Physiol 12, 380–385 (1982). https://doi.org/10.1007/BF01189299

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