Abstract
Peripheral blood lymphocytes (PBL) from rabbits of the Chbb: HM strain proliferated in coculture with an X-ray-irradiated HTLV-I-transformed leukemogenic cell line of (B/JxChbb: HM) F1 origin, whereas PBL from rabbits of the B/J and F1 strains hardly proliferated at all in co-culture with the same cell line. A proviral HTLV-I genome was detected in high-molecular-mas DNA from these proliferating cells. An analysis of T cell receptor Vß expression revealed that these lymphocytes were of restricted Vß subfamilies, suggesting that the preferential stimulation and transformation of lymphocytes occurred in this co-culture. Staphylococcal enterotoxins similarly stimulated lymphocytes and the proliferated lymphocytes were mostly of distinct Vß subfamilies depending on stimulator enterotoxins. These results suggested that the leukemogenic cell line possesses an antigen that preferentially stimulates lymphocytes of restricted Vß subfamilies
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Abbreviations
- PHA :
-
phytohemagglutinin
- PCR :
-
polymerase chain reaction
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Isono, T., Isegawa, Y. & Seto, A. Analysis of T cell receptor Vß expression in rabbit T lymphocytes induced to proliferate by an HTLV-I-transformed leukemogenic T cell line. J Cancer Res Clin Oncol 122, 458–464 (1996). https://doi.org/10.1007/BF01187157
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DOI: https://doi.org/10.1007/BF01187157