Summary
Developmental changes in relative amounts of peripheral nerve proteins and glycoproteins have been correlated with the degree of morphological myelination at various ages during the first 25 postnatal days in rat sciatic nerve. At birth there is virtually no major myelin glycoprotein (P0), but there is a protein which migrates to the same point on sodium dodecyl sulphate (SDS) polyacrylamide gels as the small myelin basic protein (P2). During the time myelin is being formed in the nerve, the P0 protein increases and the P2 protein appears to decrease in relative amount in the nerve. The accumulation of P0 protein in the nerve correlates extremely well with the degree of myelination in sciatic nerve.
At 4–6 days postnatal, smooth membrane profiles are observed which are located within axons and in the inner Schwann cell cytoplasm. Such profiles are also observed to fuse with the axolemma-Schwann cell interface. The profiles may represent membrane material being added to or deleted from the axolemma or myelin during myelination.
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References
Autilio-Gambetti, L., Gambetti, P. andShafer, B. (1975) Glial and neuronal contribution to proteins and glycoproteins recovered in myelin fractions.Brain Research 84, 336–40.
Brady, R. O. andQuarles, R. H. (1973) The enzymology of myelination.Molecular and Cellular Biochemistry 2, 23–9.
Elam, J. S. (1975) Association of proteins undergoing slow axonal transport with goldfish visual system myelin.Brain Research 97, 303–15.
Everly, J. L., Brady, R. O. andQuarles, R. H. (1973) Evidence that the major protein in rat sciatic nerve myelin is a glycoprotein.Journal of Neurochemistry 21, 329–34.
Fairbanks, G., Steck, T. L. andWallach, D. F. H. (1971) Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane.Biochemistry 10, 2606–17.
Friede, R. L. andSamorajski, T. (1968) Myelin formation in the sciatic nerve of rat. A quantitative electron microscopic histochemical and radioautographic study.Journal of Neuropathology and Experimental Neurology 27, 546–70.
Giorgi, P. P., Karlsson, J. O., Sjöstrand, J. andField, E. J. (1973) Axonal flow and myelin protein in the optic pathway.Nature, New Biology 244, 121–4.
Hendrikson, C. K. andVaughn, J. E. (1974) Fine structural relationships between neurites and radial glial processes in developing mouse spinal cord.Journal of Neurocytology 3, 659–75.
Johnson, J. D., Douvas, A. S. andBonner, J. (1974) Chromosomal Proteins.International Review of Cytology (Suppl. 4), 273–361.
Matthieu, J. M., Everly, J. L., Brady, R. O. andQuarles, R. H. (1975) [35S] Sulfate incorporation into myelin glycoproteins. II. Peripheral nervous tissue.Biochimica et Biophysica Acta 392, 167–74.
Peters, A. andVaughn, J. E. (1970) Morphology and development of the myelin sheath. InMyelination (edited byDavidson, A. N. andPeters, A.) pp: 7–37. Springfield: Thomas.
Peterson, R. G. andSea, C. P. (1975) Localization of the main myelin protein in PNS.American Society for Neurochemistry Transactions 6, 211 (abstract).
Prensky, A. L., Fujimoto, K. andAgrawal, H. C. (1975) Are myelin proteins synthesized in retinal ganglion cells?Journal of Neuro chemistry 25, 883–8.
Spencer, P. S. andThomas, P. K. (1974) Ultrastructural studies of the dying-back process II. The sequestration and removal by Schwann cells and oligodendrocytes of organelles from normal and diseased axons.Journal of Neurocytology 3, 763–83.
Webster, H. deF. (1971) The geometry of peripheral myelin sheaths during their formation and growth in rat sciatic nerves.Journal of Cell Biology 48, 348–67.
Webster, H. deF., Martin, J. R. andO'connell, M. F. (1973) The relationships between interphase Schwann cells and axons before myelination: A quantitative electron microscopic study.Developmental Biology 32, 401–16.
Wiggins, R. C., Benjamins, J. A. andMorell, P. (1975) Appearance of myelin proteins in rat sciatic nerve during development.Brain Research 89, 99–106.
Wolfgram, F. andKotorii, K. (1968) The composition of myelin proteins of the peripheral nervous sytem.Journal of Neurochemistry 15, 1281–90.
Wolman, M. (1957) Histochemical study of changes occurring during the degeneration of myelin.Journal of Neurochemistry 1, 370–6.
Wood, J. G. andDawson, R. M. C. (1973) A major myelin glycoprotein of sciatic nerve.Journal of Neurochemistry 21, 717–9.
Wood, J. G. andDawson, R. M. C. (1974a) Some properties of a major structural glycoprotein of sciatic nerve.Journal of Neurochemistry 22, 631–5.
Wood, J. G. andDawson, R. M. C. (1974b) Lipid and protein changes in sciatic nerve during Wallerian degeneration.Journal of Neurochemistry 22, 627–30.
Wood, J. G. andMclaughlin, B. J. (1975) The visualization of Concanavalin A binding sites on the interperiod line of rat sciatic nerve myelin.Journal of Neurochemistry 24, 233–5.
Wood, J. G. andSarinana, F. O. (1975) The staining of sciatic nerve glycoproteins on polyacrylamide gels with Concanavalin A-peroxidase.Analytical Biochemistry 69, 320–2.
Zgorzalewicz, B., Neuhoff, V. andWaehneldt, T. V. (1974) Rat myelin proteins. Compositional changes in various regions of the nervous system during ontogenetic development.Neurobiology 4, 265–76.
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Wood, J.G., Engel, E.L. Peripheral nerve glycoproteins and myelin fine structure during development of rat sciatic nerve. J Neurocytol 5, 605–615 (1976). https://doi.org/10.1007/BF01175573
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DOI: https://doi.org/10.1007/BF01175573