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Staining methods for an electron microscopic analysis of Golgi impregnated nervous tissue and a demonstration of the synaptic distribution upon pulvinar neurons

  • Published:
Journal of Neurocytology

Summary

A Golgi impregnation method was modified for electron microscopy by reducing the time of the staining period. An acceptable level of the ultrastructural preservation was produced in several species of animals. Various methods were examined for removing the dense silver precipitate in order to show organelles in the stained neurons. Some methods for identifying particular parts of stained neurons in electron microscopy were also investigated by observing a sectioned block under an incident light microscope.

The Golgi-EM method was applied to a study of the pulvinar nucleus in the rhesus monkey in order to demonstrate the usefulness and the limits of the method. Three neurons were studied, and at least four types of terminals (RL, F1, F2 and RS) were found synapsing on them. The distribution pattern of each type of terminal was determined upon the three neurons.

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References

  • Blackstad, T. W. (1970) Electron microscopy of Golgi preparations for study of neuronal relations. InContemporary Research Methods in Neuroanatomy (edited byNauta, W. J. H. andEbbesson, S. O. E.), pp. 186–215. Berlin: Springer.

    Google Scholar 

  • Blackstad, T. W. (1975) Golgi preparations for electron microscopy: Controlled reduction of the silver chromate by ultraviolet illumination. InGolgi Centennial Symposium: Perspectives in Neurobiology (edited bySantini, M.), pp. 123–32. New York: Raven.

    Google Scholar 

  • Boycott, B. B. andKolb, H. (1973) The connections between bipolar cells and photoreceptors in the retina of the domestic cat.Journal of Comparative Neurology 147, 91–114.

    Google Scholar 

  • Campos-Ortega, J. A. andHayhow, W. R. (1973) The synaptic organization in the inferior pulvinar of the rhesus monkey (Macaca mulatta).Brain Behavior and Evolution 7, 203–47.

    Google Scholar 

  • Chan-Palay, V. andPalay, S. L. (1972a) High voltage electron microscopy of rapid Golgi preparations. Neurons and their processes in the cerebellar cortex of monkey and rat.Zeitschrift für Anatomie und Entwicklungsge schicte 137, 125–52.

    Google Scholar 

  • Chan-Palay, V. andPalay, S. L. (1972b) The form of velate astrocytes in the cerebellar cortex of monkey and rats: High voltage electron microscopy of rapid Golgi preparations.Zeitschrift für Anatomie und Entwicklungsgeschichte 138, 1–19.

    Google Scholar 

  • Famiglietti, E. V. Jr. andPeters, A. (1972) The synaptic glomerulus and intrinsic neuron in the dorsal lateral geniculate nucleus of the cat.Journal of Comparative Neurology 144, 285–334.

    PubMed  Google Scholar 

  • Frontera, J. G. (1964) Improved Golgi-type impregnation of nerve cell.Anatomical Record 148, 371–2.

    Google Scholar 

  • Guillery, R. W. (1969) The organization of synaptic interconnections in the laminae of the dorsal lateral geniculate nucleus of the cat.Zeitschrift für Zellforschung und mikroskopische Anatomie 96, 1–38.

    Google Scholar 

  • Guillery, R. W. (1971) Patterns of synaptic interconnections in the dorsal lateral geniculate nucleus of cat and monkey: A brief review.Vision Research, Supplement No.3, 211–27.

    Google Scholar 

  • Guillery, R. W. AndColonnier, M. (1970) Synaptic patterns in the dorsal lateral geniculate nucleus of the monkey.Zeitschrift für Zellforschung und miroskopische Anatomie 103, 90–108.

    Google Scholar 

  • Ito, H. andKishida, R. (1974) A Golgi-type impregnation method tor electron microscopy.Journal für Hirnforschung 15, 409–17.

    Google Scholar 

  • Karlsson, U. (1967) Three-dimensional studies of neurons in the lateral geniculate nucleus of the rat. III Specialized neuronal contacts in the neuropil.Journal of Ultrastructure Research 17, 137–57.

    PubMed  Google Scholar 

  • Levay, S. (1973) Synaptic patterns in the visual cortex of the cat and monkey. Electron microscopy of Golgi preparations.Journal of Comparative Neurology 150, 53–86.

    PubMed  Google Scholar 

  • Levinthal, C. andWare, R. (1972) Three-dimensional reconstruction from serial sections.Nature 236, 207–10.

    Google Scholar 

  • Lieberman, A. R. (1974) Comments on the fine structural organization of the dorsal lateral geniculate nucleus of the mouse.Zeitschrift für Anatomie und Entwicklungsgeschicte 145, 261–7.

    Google Scholar 

  • Lieberman, A. R. andWebster, K. E. (1974) Aspects of the synaptic organization of intrinsic neurons in the dorsal lateral geniculate nucleus. An ultrastructural study of the normal and of the experimentally deafferented nucleus in the rat.Journal of Neurocytology 3, 677–710.

    PubMed  Google Scholar 

  • Mathers, L. H. (1971) A light and EM study of the squirrel monkey pulvinar.Anatomical Record 169, 267.

    Google Scholar 

  • Ralston, H. J. III. (1971) Evidence for presynaptic dendrites and a proposal for their mechanism of action.Nature 230, 585–7.

    PubMed  Google Scholar 

  • Ralston, H. J. III. andHerman, M. M. (1969) The fine structure of neurons and synapses in the ventrobasal thalamus of the cat.Brain Research 14, 77–97.

    PubMed  Google Scholar 

  • Ramón-Moliner, R. (1970) The Golgi-Cox technique. InContemporary Research Methods in Neuroanatomy (edited byNauta, W. J. H. andEbbesson, S. O. E.), pp. 32–55. Berlin: Springer.

    Google Scholar 

  • Ramón-Moliner, E. andFerrari, J. (1972) Electron microscopy of previously identified cells and processes within the central nervous system.Journal of Neurocytology 1, 85–100.

    PubMed  Google Scholar 

  • Reese, T. S. andShepherd, G. M. (1972) Dendrodendritic synapses in the CNS. InStructure and Function of Synapses (edited byPappas, G. D. andPurpura, D. P.), pp. 121–36. New York: Raven.

    Google Scholar 

  • Scott, G. L. andGuillery, R. W. (1974) Studies with the high voltage electron microscope of normal, degenerating, and Golgi impregnated neuronal processes.Journal of Neurocytology 3, 567–90.

    PubMed  Google Scholar 

  • Shelton, P. M. J., Horridge, G. A. andMeinertzhagen, I. A. (1971) Reconstruction of synaptic geometry and neural connections from serial thick sections examined by the medium high voltage electron microscope.Brain Research 29, 373–7.

    PubMed  Google Scholar 

  • Sjöstrand, F. S. (1958) Ultrastructure of retinal rod synapses of the guinea pig eye as related by three-dimensional reconstructions from serial sections.Journal of Ultrastructure Research 2, 122–70.

    PubMed  Google Scholar 

  • Špaček, J. andLieberman, A. R. (1974) Ultrastructure and three-dimensional organization of synaptic glomeruli in rat somatosensory thalamus.Journal of Anatomy 117, 487–516.

    PubMed  Google Scholar 

  • Stell, W. K. (1965) Correlation of retinal cytoarchitecture and ultrastructure in Golgi preparation.Anatomical Record 153, 389–97.

    PubMed  Google Scholar 

  • Stell, W. K. (1967) The structure and relationships of horizontal cells and photoreceptor-bipolar synaptic complexes in goldfish retina.American Journal of Anatomy 121, 401–24.

    PubMed  Google Scholar 

  • West, R. W. (1972) Superficial warming of epoxy blocks for cutting of 25–150 μm sections to be resectioned in the 40–90 nm range.Stain Technology 47, 201–4.

    PubMed  Google Scholar 

  • West, R. W. AndDowling, J. E. (1972) Synapses onto different morphological types of retinal ganglion cells.Science 178, 510–12.

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Anatomy, Yokohama City University, Yokohama, Japan

    Hironobu Ito

  2. Department of Anatomy, University of Wisconsin, 53706, Madison, Wisconsin, USA

    Frank Atencio

Authors
  1. Hironobu Ito
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  2. Frank Atencio
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Ito, H., Atencio, F. Staining methods for an electron microscopic analysis of Golgi impregnated nervous tissue and a demonstration of the synaptic distribution upon pulvinar neurons. J Neurocytol 5, 297–317 (1976). https://doi.org/10.1007/BF01175117

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  • DOI: https://doi.org/10.1007/BF01175117

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