Abstract
In vitro culture was used to study morphogenetic aspects of the tuberization process under controlled conditions in potato (Solanum tuberosum L.) plants. This paper accurately defines four stages of tuber development and their correlation to external morphological characteristics and histological structures. Protein kinase activity, assayed in each stage using Historic HAS as substrate, was differentially expressed during the tuberization process. Phosphorylation was maximum in the first stages of tuber formation. The incorporation of [32PO4 −1] to endogenous peptides containing serine/threonine amino acidic residues followed the same pattern that the protein kinase activity did.
Similar content being viewed by others
Abbreviations
- EDTA:
-
Ethylenediaminetetraacetic acid
- EGTA :
-
ethylenebis (oxyethylenenitrilo) tetraacetic acid
- MOPS :
-
4-morpholine-propanesulfonic acid
References
Cutter EG (1978) Structure and development of the potato plant. In: Harris PM (ed) The potato crop. Chapman and Hall, London, pp 70–152
Ewing EE (1987) The role of hormones in potato (Solanum tuberosum L.) tuberization. In: Davies PJ (ed) Plant hormones and their role in plant growth and development. Kluwer Academic Publ, Dordrecht, pp 515–538
Felix G, Grosskopf DG, Regenass M, Boller T (1991) Rapid changes of protein phosphorylation are involved in transduction of the elicitor signal in plant cells. Proc Natl Acad Sci USA 88: 8831–8834
Grosskopf DG, Felix G, Boller T (1990) K-252a inhibits the response of tomato cells to fungal elicitors in vivo and their microsomal protein kinase in vitro. FEBS Lett 275:177–180
Jensen WA (1962) Botanical histochemistry. Principles and practice. Freeman & Co, San Francisco
Lowry OH, Rosebrough NJ, Farr RL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193: 265–275
Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15: 473–497
Reymond P, Short TW, Briggs WR, Poff KL (1992) Light-induced phosphorylation of a membrane protein plays an early role in signal transduction for phototropism inArabidopsis thaliana. Proc Natl Acad Sci USA 89:4718–4721
Roskoski RJr (1983) Assays of protein kinases. Methods Enzymol 99:3–6
Stone JM, Walker JC (1995) Plant protein kinase families and signal transduction. Plant Physiol 108:451–457
Taylor MA, Mad Arif SA, Kumar A, Davies HV, Scobie LA, Pearce SR, Flavell AJ (1992a) Expression and sequence analysis of cDNAs induced during the early stages of tuberisation in different organs of the potato plant (Solanum tuberosum L.) Plant Mol Biol 20:641–651
Taylor MA, Mad Arif SA, Pearce SR, Davies HV, Kumar A, George LA (1992b) Differential expression and sequence analysis of ribosomal protein genes induced in stolon tips of potato (Solanum tuberosum L.) during the early stages of tuberization. Plant Physiol 100:1171–1176
Tovar P, Estrada R, Schilde-Renschler L, Dodds JH (1985) Induction and use of in vitro tubers. CIP circular 13:1–5
Ulloa RM, Glikin GC, Té1lez-Iñón MT, Torres HN, Judewicz ND (1987) A novel stimulator of protein phosphorylation inNeurospora crassa. Mol Cell Biochem 77:11–17
Author information
Authors and Affiliations
Additional information
Communicated by R. N. Trigiario
Rights and permissions
About this article
Cite this article
Ulloa, R.M., Mac Intosh, G.C., Melchiorre, M. et al. Protein kinase activity in different stages of potato (Solanum tuberosum L.) microtuberization. Plant Cell Reports 16, 426–429 (1997). https://doi.org/10.1007/BF01146787
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01146787