Abstract
In leaf discs ofSaintpaulia ionantha xconfusa hybrid (cv. Virginia) cultured on shoot-inducing medium, periclinal divisions were initiated in epidermal cells 3–5 days after explant isolation. This timing coincided with the time for competence acquisition determined in tissue-transfer experiments. Some of the daughter cells from periclinal divisions formed the target cells which divided both anticlinally and periclinally to form cell division centers (meristemoids), precursors of adventitious shoots. The target cells were not morphologically distinct from other epidermal cells at the light microscope level. It is suggested that the periclinal divisions in epidermal cells represent the dedifferentiation phase during which target (competent) cells are formed. Once the cells have acquired the ability to divide periclinally, both dedifferentiation and shoot induction occur in the presence of exogenous plant hormones.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- SIM :
-
Shoot-inducing medium
References
Attfield EM, Evans PK (1991) Stages in the initiation of root and shoot organogenesis in cultured leaf explants ofNicotiana tabacum cv. Xanthi nc. J Exp Bot 42:59–63
Barcelo P, Lazzeri PA, Martin A, Lorz H (1991) Competence of cereal leaf cells. I. Patterns of proliferation and regeneration capability in vitro of the inflorescence sheath leaves of barley, wheat andtritordeum. Plant Sci 77:243–251
Chlyah H, Tran Thanh Van M, Demarly Y (1975) Distribution of cell division centers on the epidermis of stem segments ofTorenia fournieri duringde novo bud formation. Plant Physiol 56:28–33
Christianson ML, Warnick DA (1983) Competence and determination in the process ofin vitro shoot organogenesis. Dev Biol 95:288–293
Dolezelova M, Dolezel J, Nesticky M (1992) Relationship of embryogenic competence in maize (Zea mays L.) leaves to mitotic activity, cell cycle and nuclear DNA content. Plant Cell Tissue Organ Cult 31:215–221
Finstad K, Brown DCW, Joy K (1993) Characterization of competence during induction of somatic embryogenesis in alfalfa tissue culture. Plant Cell Tissue Organ Cult 34:125–132
Halperin W (1973) The use of cultured tissue in studying developmental problems. Can J Bot 51:1801–1806
Joarder OI, Joarder NH, Dale PJ (1986) In vitro response of leaf tissues fromLolium multiforum — a comparison with leaf segment position, leaf age and in vitro mitotic activity. Theor Appl Genet 73:286–291
Johansen DA (1940) Plant Microtechnique, Waltham, Mass
Lo KH, Giles KL, Sawhney VK (1997) Acquisition of competence for shoot regeneration in leaf discs ofSaintpaulia ionantha xconfusa hybrids (African violet) cultured in vitro. Plant Cell Rep 416–420
McDaniel CN (1984) Competence, determination, and induction in plant development. In: Malacinski GM, Bryant SV (eds) Pattern formation, MacMillan New York, pp 393–412
Meins F Jr, Binns AN (1979) Cell determination in plant development. BioScience 29:221–225
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497
Naylor EH, Johnson B (1937) A histological study of vegetative reproduction inSaintpaulia ionantha. Am J Bot 24:673–678
Ohki S (1994) Scanning electron microscopy of shoot differentiation in vitro from leaf explants of the African violet. Plant Cell Tissue Organ Cult 36:157–162
Redway FA (1991) Histology and stereological analysis of shoot formation in leaf callus ofSaintpaulia ionantha Wendl. (African violet). Plant Sci 73:243–251
Thorpe TA (1980) Organogenesis in vitro: structural, physiological, and biochemical aspects. Int Rev Cytol [suppl II A]:71–111
Torrey JG (1977) Cytodifferentiation in cultured cells and tissues. HortScience 12:14–15
Tran Thanh Van K (1981) Control of morphogenesis in in vitro cultures. Annu Rev Plant Physiol 32:291–311
Vazquez AM, Short KC (1978) Morphogenesis in cultured floral parts of African violet. J Exp Bot 29:1265–1271
Author information
Authors and Affiliations
Additional information
Communicated by F. Constabel
Rights and permissions
About this article
Cite this article
Lo, K.H., Giles, K.L. & Sawhney, V.K. Histological changes associated with acquisition of competence for shoot regeneration in leaf discs ofSaintpaufla ionantha xconfusa hybrid (African violet) cultured in vitro. Plant Cell Reports 16, 421–425 (1997). https://doi.org/10.1007/BF01146786
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01146786