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Factors affectingAgrobacterium tumefaciens-mediatedgusA expression and opine synthesis inGladiolus

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Abstract

Five tumorigenic strains ofAgrobacterium tumefaciens were used to inoculate corms, cormels, plants grown in vitro, and seed-derived seedlings of several cultivars ofGladiolus. Tumors formed on 12% of the plant tissues inoculated, and 1% of these tumors synthesized either octopine or nopaline.A. tumefaciens-mediatedβ-glu-curonidase (GUS) expression showed 0.5% and 3.5% GUS expression for plants grown in vitro and regenerable callus, respectively. GUS expression ranged from 40% to 61% whenA. tumefaciens was incubated with leaves from seedlings grown in the dark, whereas leaves from seedlings grown under a 16-h light photoperiod showed no GUS, indicating the significant effect of etiolation on transient GUS expression mediated byA. tumefaciens.

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Authors and Affiliations

  1. United States Department of Agriculture National Arboretum, Floral and Nursery Plants Research Unit, B-010A Room 238, BARC West, 20705-2350, Beltsville, MD, USA

    K. Kamo

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  1. K. Kamo
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Communicated by R. N. Trigiano

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Kamo, K. Factors affectingAgrobacterium tumefaciens-mediatedgusA expression and opine synthesis inGladiolus . Plant Cell Reports 16, 389–392 (1997). https://doi.org/10.1007/BF01146779

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  • DOI: https://doi.org/10.1007/BF01146779

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