Abstract
An efficient and reproducible procedure is described for the large-scale propagation of an epiphytic orchid,Acampe praemorsa (Roxb.) B latter and McCann using foliar explants. Shoot buds were induced in basal parts of foliar explants on Murashige and Skoog medium supplemented with N6-benzyladenine (BA), kinetin (Kn) or thidiazuron (TDZ), the latter being most effective at 1.0 mg/1. Shoots formed to a TDZ-containing medium elongated following transfer to a substrate supplemented with 2.0 mg/l 1-naphthaleneacetic acid (NAA) and 0.5 mg/1 BA. NAA at lower concentrations had no beneficial effects on shoot regeneration, whether added to the medium along with BA, Kn or TDZ. However, it promoted shoot elongation and leaf expansion. Higher concentrations of NAA suppressed shoot regeneration. The frequency of shoot regeneration was greatly influenced by the developmental stage and orientation of the leaf. Shoots regenerated from the foliar explants were rooted successfully on MS medium containing 1.0 mg/l indole-3-butyric acid. The plantlets were acclimated and eventually transferred to a garden.
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Abbreviations
- BA :
-
N6-Benzyladenine
- IAA :
-
Indole-3-acetic acid
- IBA :
-
Indole-3-butyric acid
- Kn :
-
Kinetin
- MS :
-
Murashige and Skoog (1962) medium
- NAA :
-
1-Naphthaleneacetic acid
- TDZ :
-
Thidiazuron (N-phenyl-N′-1,2,3-thiadiazol-5-ylurea)
References
Arditti J, Ernst R (1993) Micropropagation of orchids. Wiley, New York
Churchill ME, Ball EA, Arditti J (1970) Production of orchid plants from seedling leaf tips. Orchid Dig 34:271–273
Churchill ME, Arditti J, Ball EA (1971) Clonal propagation of orchids from leaf tips. Am Orchid Soc Bull 40:109–113
Ernst R (1994) Effects of thidiazuron on in vitro propagation ofPhalaenopsis andDoritaenopsis (Orchidaceae). Plant Cell Tissue Organ Cult 39:273–275
Gob CJ (1982) Development of orchid tissue culture in South East Asian countries. In: Rao AN (ed) Tissue culture of economically important plants. COSTED/ANBS, Singapore, pp 205–209
Huetteman CA, Precee JE (1993) Thidiazuron: a potent cytokinin for woody plant tissue culture. Plant Cell Tissue Organ Cult 33:105–119
Jain SK, Mehrotra A (1984) A preliminary inventory of Orchidaceae in India. Botanical Survey of India, Calcutta
Jaiwal PK, Gulati A (1991) In vitro high frequency plant regeneration of a tree legume,Tamarindus indica L. Plant Cell Rep 10:569–573
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473–497
Precee JE, Imel MR (1991) Plant regeneration from leaf explants ofRhododendron ‘P.J.M. hybrids’. Sci Hort 48:159–170
Rao AN (1977) Tissue culture in orchid industry. In: Reinert J, Bajaj YPS (eds) Applied and fundamental aspects of plant cell, tissue and organ culture. Springer, Berlin Heidelberg New York, pp 44–69
Sagawa Y, Kunisaki JT (1984) Clonal propagation: orchids. In: Vasil IK (ed) Cell culture and somatic cell genetics of plants, vol 1. Academic Press, London, pp 61–67
Seeni S, Latha PG (1992) Foliar regeneration of the endangered Red Vanda,Renanthera imschootiana. Plant Cell Tissue Organ Cult 29:167–172
Vij SP, Sood A, Plaha KK (1984) Propagation ofRhynchostylis retusa B1 (Orchidaceae) by direct organogenesis from leaf segment cultures. Bot Gaz 145:210–214
Willis JC (1973) A dictionary of flowering plants and ferns. Cambridge University Press, Cambridge, UK
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Nayak, N.R., Patnaik, S. & Rath, S.P. Direct shoot regeneration from foliar explants of an epiphytic orchid,Acampe praemorsa (Roxb.) Blatter and McCann. Plant Cell Reports 16, 583–586 (1997). https://doi.org/10.1007/BF01142328
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DOI: https://doi.org/10.1007/BF01142328