Abstract
Somatic embryogenesis was achieved in callus cultures dervied from 40-day-old semimature zygotic embryos ofDalbergia sissoo on semi-solid Murashige and Skoog (MS) salts and vitamins supplemented with 0.46–1.16 μM kinetin, 6.78–9.04 μM 2,4-dichlorophenoxy acetic acid (2,4-D) and 30 g/1 sucrose. Somatic embryos proliferated rapidly by secondary somatic embryogenesis after transfer to half-strength basal MS medium supplemented with 0.46-1.16 μM kinetin and 6.78–9.04 μM 2,4-D with 2% (w/v) sucrose. The light-green somatic embryos germinated on half-strength MS salts and vitamins supplemented with 0.5 mg/1 abscisic acid and 2% (w/v) sucrose. The developmental stages of somatic embryogenesis were studied by light and scanning electron microscopy.
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Abbreviations
- ABA :
-
Abscisic acid
- BA :
-
6-benzyladenine
- Kn :
-
kinetin
- 2,4-D :
-
2,4-dichlorophenoxyacetic acid
- NAA :
-
1-naphthaleneacetic acid
- MS :
-
Murashige and Skoog (1962) basal medium
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Das, P., Samantaray, S., Rout, G.R. et al. In vitro somatic embryogenesis ofDalbergia sissoo Roxb. —a multipurpose timber-yielding tree. Plant Cell Reports 16, 578–582 (1997). https://doi.org/10.1007/BF01142327
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DOI: https://doi.org/10.1007/BF01142327