Abstract
Oocyte recovery in an in vitro fertilization and embryo transfer (IVF-ET) program can be performed by laparoscopy or by sonography. Most IVF teams use either one or the other of these methods. In Clamart, we undertook 407 IVF attempts from September 1983 to March 1984, 225 by laparoscopy under general anesthesia, 97 by laparoscopy under local anesthesia, and 85 by sonography. Excluding the recovery techniques, all aspects of the program (ovulation monitoring, culture, and transfer) were identical in order to study the effects of the anesthesia and the ultrasound. We did not find any significant difference in pregnancy rates, inclusive of biochemical pregnancies, among these three recovery methods, although ultrasonically guided puncture gave fewer follicles, oocytes, and embryos than laparoscopy.
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Edwards RG, Donahue RP, Baramki TA, Jones JW: Preliminary attempts to fertilize human oocytes matured in vitro. Am J Obstet Gynecol 1966;96:192–200
Steptoe PC, Edwards RG: Laparoscopic recovery of preovulatory human oocytes after priming of ovaries with gonadotrophins. Lancet 1970;1:683–689
Semm K: Incidents et accidents de la coelioscopie.In Atlas de Coelioscopie et d'Hystéroscopie. Paris, Masson, 1977, pp 15–29
Frenkel Y, Oelsner G, Ben-Baruch G, Menczer J: Major surgical complication of laparoscopy. Eur J Obstet Gynecol Reprod Biol 1981;12:107–111
Belaisch-Allart JC, Guillet-Rosso F, Baton C, Champagne C, Bodereau AM, Frydman R: Coelioscopie sous anesthésie locale pour recueil d'ovocyte en vue d'une fécondation in vitro. Nouv Presse Med 1983;12:2053–2054
Lenz S, Lauritsen JG: Ultrasonically guided percutaneous aspiration of human follicles under local anesthesia: A new method of collecting oocytes for in vitro fertilization. Fertil Steril 1982;38:673–677
Wikland M, Nilsson L, Hansson R, Hamberger L, Janson PO: Collection of human oocytes by the use of sonography. Fertil Steril 1983;39:603–608
Roger R., Grenier J., Houlbert C, Castanier M, Feinstein MC, Scholler R: Rapid radioimmunoassay of plasma LH and oestradiol-17-B for the prediction of ovulation. J Steroids Biochem 1980;12:403–410
Frydman R, Feinstein MC, Testart J, Thebault A, Labbe A, Grenier J, Roger M, Scholler R: Recueil d'ovocytes humains en phase préovulatoire. Intérêt du dosage radioimmunologique rapide de la LH plasmatique. J Gynecol Obstet Biol Reprod 1981;10:127–132
Hazout A, Guillet-Rosso F, Belaisch-Allart JC, Glissant M, Porchier J, Testart J, Frydman R: Technique et résultat de recueil d'ovocytes humains sous échoguidage pour fécondation in vitro. Gynécologie 1984;35:265–269
Testart J, Lassale B, Frydman R, Belaisch-Allart JC: A study of factors affecting the success of human fertilization in vitro. II. Influence of semen quality and oocyte maturity on fertilization and cleavage. Biol Reprod 1983;28:425–431
Testart J, Belaisch-Allart JC, Hazout A, Guillet-Rosso F, Frydman R: Actual method and results in an IVF and ET program. Vth Reinier de Graaf Symposium, Nijmegen, August 23–25, 1984
Dellenbach P, Nissand I, Moreau L, Feger P, Plumese C, Gerlinger P, Brun B, Rumpler Y: Prelevement ovocytaire transvaginal echoguidé. Fécondation in vitro et transfert d'embryons. Bilan de nos premières reussites. Presented at 4ème Symposium de la Société Franco-Allemande de Gynecologie et Obstétrique, Paris, April 26–29, 1984
Szalay S, Feichtinger W, Kemeter P, Beck A, Janish H, Neumark J: Changes in hormonal parameters under different kinds of general anaesthetics during laparoscopy oocyte recovery.In Human Conception in Vitro, RG Edwards, J Purdy (eds). New York, Academic Press, 1982, pp 105–117
Forman R, Fishel SB, Waters D, Edwards RG: Unpublished data
Yamaguchi DT, Vaupel R: Ultrasonic effects on the developing chick embryo. Anat Rec 1978;190:588–590
Childs S, Carsteinsew E, Smachlo K: Effects of ultrasound on drosophila. I. Killing of eggs exposed to travelling and standing wave fields. Ultrasound Med Biol 1980;6:127–130
Puissant F, Lejeune F, Leroy F: Effects on mature mouse oocytes of ultrasound treatment applied before in vitro fertilization. IRCS Med Sci 1984;12:421–422
Testart J, Thebault A, Souderes E, Frydman R: Premature ovulation after ovarian ultrasonography. Br J Obstet Gynaecol 1982;89:694
Demoulin A, Shaaps JP, Bologne R, Lambotte R: Is ultrasound monitoring of follicular growth harmless? J Vitro Fert Embryo Transfer 1984;1:106
Serr D, Dor J, Shalev J, Rudak E: Unpublished data
Wikland M, Enk L, Hamberger L: Transvesical and transvaginal approaches for the aspiration of follicles by use of ultrasound. J Vitro Fert Embryo Transfer 1984;1:150
Degueldre M, Puissant F, Camus M, Tomberg A, Phan-Thi H, Melot L, Leroy F: Effects of carbon dioxide insufflation at laparoscopy on the gas phase in oocyte recovery fluids. J Vitro Fert Embryo Transfer 1984;1:106
Lefevre B, Frydman R, Belaisch-Allart J, Guillet-Rosso F, Testart J: Ovarian response to stimulation treatment in an in vitro fertilization and embryo transfer program. Vth Reinier de Graaf Symposium, Nijmegen, August 23–25, 1984
Kemeter P, Feichtinger W, Nemark J, Szalay S, Bieglmayer Ch, Janish H: Influence of laparoscopic follicular aspiration under general anaesthesia on corpus luteum, progesterone, secretion in normal and clomiphene stimulated cycle. Br J Obstet Gynaecol 1982;89:948–950
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Belaisch-Allart, J.C., Hazout, A., Guillet-Rosso, F. et al. Various techniques for oocyte recovery in an in vitro fertilization and embryo transfer program. J Assist Reprod Genet 2, 99–104 (1985). https://doi.org/10.1007/BF01139341
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DOI: https://doi.org/10.1007/BF01139341