Skip to main content
SpringerLink
Log in

Common action of certain viruses, toxins, and activated complement: pore formation and its prevention by extracellular Ca2+

  • Published:
Bioscience Reports

Abstract

Haemolysis by Sendal virus, α-toxin, and activated complement is inhibited by high concentrations of divalent cations. In Daudi cells, sublytic amounts of these agents induce the following changes: collapse of surface membrane potential, uptake of Na+ and loss of K+ from cells, and leakage of phosphorylated metabo-tites from cells. The changes induced by Sendal virus and complement are sensitive to physiological concentrations of extracellular Ca2+. It is concluded that fluctuations in plasma Ca2+ concentration may affect the damaging action of certain pore-forming agents on susceptible cells.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Arbuthnott JP (1982) In: Molecular Action of Toxins and Viruses (Cohen & S van Heyningen, eds), pp 107–129, Elsevier Biomedical Press.

  • Baez M, Palese P & Kilbourne ED (1980) J. Infect. Diseases141 362–365.

    Google Scholar 

  • Bashford CL & Pasternak CA (1984) J. Membrane Biol.79, 275–284.

    Google Scholar 

  • Bashford CL, Alder G, Micklem KJ & Pasternak CA (1983a) Biosci. Rep.3, 631–642.

    PubMed  Google Scholar 

  • Bashford CL, Micklem KJ & Pasternak CA (1983b) J. Physiol.343 100–101 P.

    Google Scholar 

  • Bhakdi S & Tranum-Jensen J (1983) Biochim. Biophys. Acta737, 342–372.

    Google Scholar 

  • Bhadki S & Tranum-Jensen J (1984) Phil. Trans. Roy. Soc., in press.

  • Campbell AK, Daw RA & Luzio JP (1979) FEBS Lett.107, 55–60.

    PubMed  Google Scholar 

  • Campbell AK, Daw RA, Hallett MB & Luzio JP (1981) Biochem. J.194, 551–560.

    PubMed  Google Scholar 

  • Burnet FM (1949) Nature164, 1008.

    PubMed  Google Scholar 

  • Cockroft S & Gomperts BD (1980) Biochem. J.188, 789–798.

    PubMed  Google Scholar 

  • Foster KA, Gill K, Micklem KJ & Pasternak CA (1980) Biochem. J.190, 639–646.

    PubMed  Google Scholar 

  • Forda SR, Gillies G, Kelly JS, Micklem KJ & Pasternak CA (1982) Neurosci. Lett.19, 237–242.

    Google Scholar 

  • Fuchs P, Gruder E, Gitelman J & Kohn A (1980) J. Cell Physiol.103, 271–278.

    PubMed  Google Scholar 

  • Fukai K & Suzuki T (1955) Med. J. Osaka Univ.6, 1–15.

    Google Scholar 

  • Fussle R, Bhakdi S, Sziegoleit A, Tranum-Jensen J, Kranz T & Wellensiek HJ (1981) J. Cell Biol.91, 83–94.

    PubMed  Google Scholar 

  • Getz D, Gibson JF, Sheppard RN, Micklem KJ & Pasternak CA (1979) J. Membrane Biol.50, 311–329.

    Google Scholar 

  • Gewert DR, Moore G & Clemens MJ (1983) Biochem. J.214, 983–990.

    PubMed  Google Scholar 

  • Gomperts BD, Baldwin JM & Micklem KJ (1983) Biochem. J.210, 737–745.

    PubMed  Google Scholar 

  • Hallett MB, Fuchs P & Campbell AK (1982) Biochem. J.206, 671–674.

    PubMed  Google Scholar 

  • Heisler S, Reisine TD, Hook VYH & Axelrod J (1982) Proc. Natl. Acad. Sci. U.S.A.79, 6502–6506.

    PubMed  Google Scholar 

  • Housley GM & Born GVR (1983) Proc. Roy. Soc. Lond. B219, 83–90.

    Google Scholar 

  • Huang RTC, Rott R & Klenk H-D (1981) Virol.110, 243–247.

    Google Scholar 

  • Impraim CC, Micklem KJ & Pasternak CA (1979) Biochem. Pharmacol.28, 1963–1969.

    PubMed  Google Scholar 

  • Impraim CC, Foster KA, Micklem KJ & Pasternak CA (1980) Biochem. J.186, 847–860.

    PubMed  Google Scholar 

  • Kabat EA & Mayer MM (1961) Experimental Immunochemistry, pp 133–240, Charles Thomas, Springfield, Illinois.

    Google Scholar 

  • Kagan BL (1983) Nature302, 709–711.

    PubMed  Google Scholar 

  • Klein E, Klein G, Nadkarni JS, Nadkarni JJ, Wigzell H & Clifford P (1967) Cancer Res.28, 1300–1310.

    Google Scholar 

  • Knutton S (1978) Micron9, 133–154.

    Google Scholar 

  • Lachmann PJ & Hobart MJ (1978) In: Handbook of Experimental Immunology (Weir DM, ed), pp 5A1–5A23, 3rd ed., Blackwell Scientific Publications, Oxford.

    Google Scholar 

  • Lancet Editorial (1984) Lanceti, 1161–1162.

  • Lenard J & Miller DK (1981) Virol.110, 479–482.

    Google Scholar 

  • McLaughlin SG, Szabo G & Eisenman G (1971) J. Gen. Physiol.58, 667–687.

    PubMed  Google Scholar 

  • Maeda T & Ohnishi S-I (1980) FEBS Lett.122, 283–287.

    PubMed  Google Scholar 

  • Mayer MM (1972) Proc. Natl. Acad. Sci. U.S.A.69, 2954–2959.

    PubMed  Google Scholar 

  • McNiven AC, Owen P & Arbuthnott JP (1972) J. Med. Microbiol.5, 113–122.

    PubMed  Google Scholar 

  • Mollby R (1978) In: Bacterial Toxins and Cell Membranes (Jelaszewicz J & Wadstrom T, eds), pp 367–424, Academic Press London.

    Google Scholar 

  • Morgan NG & Montague W (1984) Biosci. Rep.4, in press.

  • Muller-Eberhard HJ (1984) Phil. Trans. Roy. Soc., in press.

  • Pasternak CA (1981) Biochem. Soc. Trans.9, 14P.

  • Pasternak CA & Micklem KJ (1973) J. Membrane Biol.14, 293–303.

    Google Scholar 

  • Pasternak CA & Micklem KJ (1974) Biochem. J.140, 405–411.

    PubMed  Google Scholar 

  • Pasternak CA, Strachan E, Micklem KJ & Duncan J (1976) In: Cell Surfaces and Malignancy (Mora PT, ed), no. 28, pp 147–151, US Government Printing Office, Washington DC, Fogarty International Center Proceedings.

    Google Scholar 

  • Patel K & Pasternak CA (1983) Biosci. Rep.3, 749–755.

    PubMed  Google Scholar 

  • Poste G & Pasternak CA (1978) Cell Surface Reviews5, 306–349.

    Google Scholar 

  • Ramm LE & Mayer MM (1980) J. Immunol.124, 2281–2285.

    PubMed  Google Scholar 

  • Ramm LE, Whitlow MB & Mayer MM (1982) J. Immunol.129, 1143–1146.

    PubMed  Google Scholar 

  • Sato SB, Kawasaki K & Ohnishi S-I (1983) Proc. Natl. Acad. Sci. U.S.A.80, 3153–3157.

    PubMed  Google Scholar 

  • Schein SJ, Kagan BL & Finkelstein A (1978) Nature276, 159–163.

    PubMed  Google Scholar 

  • Sims PJ & Lauf PK (1980) J. Immunol.125, 2617–2625.

    PubMed  Google Scholar 

  • Thelestam M & Mollby R (1980) In: Natural Toxins (Eaker D & Wadstrom T, eds), pp 201–207, Pergamon Press, Oxford.

    Google Scholar 

  • Tosteson MT & Tosteson DT (1981) Biophys. J.36, 109–116.

    PubMed  Google Scholar 

  • Vaananen P & Kaariainen L (1979) J. Gen. Virol.43, 593–601.

    PubMed  Google Scholar 

  • Wyke AM, Impraim CC, Knutton S & Pasternak CA (1980) Biochem. J.190, 625–638.

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biochemistry, St. George's Hospital Medical School, Cranmer Terrace, SW17 ORE, London, UK

    C. L. Bashford, G. M. Alder, K. Patel & C. A. Pasternak

Authors
  1. C. L. Bashford
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. G. M. Alder
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. K. Patel
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. C. A. Pasternak
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Bashford, C.L., Alder, G.M., Patel, K. et al. Common action of certain viruses, toxins, and activated complement: pore formation and its prevention by extracellular Ca2+ . Biosci Rep 4, 797–805 (1984). https://doi.org/10.1007/BF01128822

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01128822

Keywords

Search

Navigation