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Islet cell surface antibodies in genetically obese hyperglycaemic (ob/ob) mice

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Bioscience Reports

Abstract

A quantitative method for circulating islet cell surface antibodies (ICSA), based on the binding of125I-protein A to insulin-producing RINm5F cells, was used to evaluate ICSA in plasma of 4- to 40-week-old Aston obese hyperglycaemic (ob/ob) mice and normal control (+/+) mice. RINm5F cells bound 2502±l196 c.p.m.125I-protein A per l05 cells (mean±S.D.,n=54) after incubation with +/+ plasma. ICSA positive plasma (defined as125I-protein A binding, mean±2 S.D. of +/+ plasma) was detected in 3 out of 54+/+ mice and 3 out of 54 ob/ob mice. ICSA were not observed in ob/ob mice before the onset of diabetes (7 weeks of age), but were detected at 9, 20 and 40 weeks. At 20 weeks125I-protein A binding produced by ob/ob plasma was 35% greater than +/+ plasma (P<0.05). The low occurrence of ICSA in ob/ob mice (6%) suggests that factors other than ICSA are responsible for B-cell dysfunction and eventual islet degeneration observed in Aston ob/ob mice.

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Authors and Affiliations

  1. Department of Biochemistry, University of Surrey, Guildford, UK

    Peter R. Flatt

  2. Metabolic Unit, Western General Hospital, Edinburgh, UK

    Adrian J. Bone

  3. Department of Molecular Sciences, Aston University, Birmingham, UK

    Clifford J. Bailey

Authors
  1. Peter R. Flatt
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  2. Adrian J. Bone
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  3. Clifford J. Bailey
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Flatt, P.R., Bone, A.J. & Bailey, C.J. Islet cell surface antibodies in genetically obese hyperglycaemic (ob/ob) mice. Biosci Rep 5, 715–720 (1985). https://doi.org/10.1007/BF01119868

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  • DOI: https://doi.org/10.1007/BF01119868

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