Skip to main content
SpringerLink
Log in

Catalysis by protein disulphide-isomerase of the assembly of trimeric procollagen from procollagen polypeptide chains

  • Published:
Bioscience Reports

Abstract

Type-I procollagen,14C-biosynthetically labelled, was reduced under denaturing and non-denaturing conditions. Reoxidation to disulphide-linked trimers occurred with non-denatured chains in the presence of an oxidant system containing oxidized and reduced glutathione. Dimeric intermediates were not detected. This reoxidation was accelerated by homogeneous beef liver protein disulphide-isomerase.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Bachinger HP, Bruckner P, Timpl R & Engel J (1978) Eur. J. Biochem.90, 605–613.

    Google Scholar 

  • Brockway BE, Forster SJ & Freedman RB (1980) Biochem. J.191, 873–876.

    Google Scholar 

  • Bruckner P, Eikenberry E & Prockop DJ (1981) Eur. J. Biochem.118, 607–613.

    Google Scholar 

  • Creighton TE, Hillson DA & Freedman RB (1980) J. Mol. Biol.142, 43–62.

    Google Scholar 

  • Dehm P & Prockop DJ (1971) Biochim. Biophys. Acta240, 358–369.

    Google Scholar 

  • Ellman G (1959) Arch. Biochem. Biophys.82, 70–77.

    Google Scholar 

  • Epstein CJ, Goldberger RF & Anfinsen CB (1963) Cold Spring Harbor Symp. Quant. Biol.28, 439–449.

    Google Scholar 

  • Freedman RB & Hillson DA (1980) in: The Enzymology of Post-Translational Modification of Proteins (Freedman RB & Hawkins HC, eds), vol 1, pp 157–212, Academic Press, London.

    Google Scholar 

  • Freedman RB, Brockway BE, Forster SJ, Lambert N, Mills ENC & Roden LT (1983) in: Functions of Glutathione: Biochemical, Physiological, Toxicological and Clinical Aspects (Larsson A, Orrenius S, Holmgren A & Mannervik B, eds), pp 271–282, Raven Press, New York.

    Google Scholar 

  • Harwood R & Freedman RB (1978) FEBS Lett.88, 46–48.

    Google Scholar 

  • Kosen PA & Franzblau C (1982) Biochemistry21, 4278–4284.

    Google Scholar 

  • Laemmli UK (1970) Nature (London)227, 680–685.

    Google Scholar 

  • Lambert N & Freedman RB (1983a) Biochem. J.213, 225–234.

    Google Scholar 

  • Lambert N & Freedman RB (1983b) Biochem. J.213, 235–243.

    Google Scholar 

  • Myllyla R, Koivu J, Pihlajaniemi T & Kivirikko KI (1983) Eur. J. Biochem.134, 7–11.

    Google Scholar 

  • Olsen BR (1982) in: Cell Biology of Extracellular Matrix (Hay ED, ed), pp 139–177, Plenum Press, New York.

    Google Scholar 

  • Prockop DJ, Kivirikko KI, Tuderman L & Guzman NA (1979) New. Engl. J. Med.301, 13–23 and 77–85.

    Google Scholar 

Download references

Author information

Author notes

  1. Simon J. Forster

    Present address: Department of Biochemistry, University of Leicester, LE1 7RH, Leicester, UK

Authors and Affiliations

  1. Biological Laboratory, University of Kent, CT2 7NJ, Canterbury, Kent, UK

    Simon J. Forster & Robert B. Freedman

Authors
  1. Simon J. Forster
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Robert B. Freedman
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Forster, S.J., Freedman, R.B. Catalysis by protein disulphide-isomerase of the assembly of trimeric procollagen from procollagen polypeptide chains. Biosci Rep 4, 223–229 (1984). https://doi.org/10.1007/BF01119657

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01119657

Keywords

Search

Navigation