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In vitro transcription of viroid RNA into full-length copies by RNA-dependent RNA polymerase from healthy tomato leaf tissue

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Bioscience Reports

Abstract

RNA-dependent RNA polymerase from healthy tomato plant tissue accepts potato spindle tuber viroid (PSTV) RNA as a template for the in vitro synthesis of full-length RNA copies of the PSTV genome. Viroid transcription requires the presence of Mn2+ and/or Mg2+ ions and is not inhibited by concentrations of l0−5 M α-amanitin. This is the first report of a well-defined product synthesized in vitro by an RNA-dependent RNA polymerase from healthy plants.

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Authors and Affiliations

  1. Arbeitsgruppe Pflanzenvirologie, Justus-Liebig-Universität, D-6300, Giessen, FRG

    Frank Boege, Wolfgang Rohde & Heinz L. Sänger

  2. Max-Planck-Institut für Biochemie, D-8033, Martinsried bei München, FRG

    Heinz L. Sänger

Authors
  1. Frank Boege
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  2. Wolfgang Rohde
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  3. Heinz L. Sänger
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Boege, F., Rohde, W. & Sänger, H.L. In vitro transcription of viroid RNA into full-length copies by RNA-dependent RNA polymerase from healthy tomato leaf tissue. Biosci Rep 2, 185–194 (1982). https://doi.org/10.1007/BF01116382

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  • DOI: https://doi.org/10.1007/BF01116382

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