Abstract
The electrical response to the synthetic auxin 1-naphthaleneacetic acid (1-NAA) ofNicotiana plumbaginifolia wild type and the monogenic, dominant auxinresistant mutant R25 was studied. Membrane potentials were continuously recorded in hypocotyl cells of light-grown, intact seedlings, and the time course of the response to 1-NAA addition was followed. Wild-type cells responded to ⩾ 10−5 M 1-NAA with a delayed, transient hyperpolarization. The R25 cells hyperpolarized significantly only in response to 1-NAA at 10−3 M, and with maximal amplitudes lower than those recorded with the wild type. In contrast, the two genotypes reacted similarly in terms of kinetics and amplitude to 10−5 M fusicoccin, which rapidly and strongly hyperpolarized the cells, and to 10−3 M benzoic acid, which induced rapid and weak hyperpolarization. The resting membrane potentials of the wild type and R25 were also not significantly different. Unlike wild-type hypocotyls, those of R25 ceased elongating before the time chosen for the electrophysiological measurements, but control experiments performed at a time when the elongation of both genotypes had terminated indicated that the difference in electrical response to auxin is independent of hypocotyl growth. The inefficiency of 1-NAA in inducing hyperpolarization of R25 hypocotyl cells suggests a defect at an early step in auxin action.
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Abbreviations
- 1-NAA:
-
1-naphthaleneacetic acid
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The authors are grateful to Prof. Jean Guern and Dr. Michael Schultze (ISV, CNRS) for helpful discussions and critically reading the manuscript, and to Philippe Muller (ISV, CNRS) for computer scanning of the membrane-potential recordings. Fusicoccin was kindly provided by Dr. A. Ballio, University of Rome, Italy.
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Stirnberg, P., King, P.J. & Barbier-Brygoo, H. An auxin-resistant mutant ofNicotiana plumbaginifolia Viv. is impaired in 1-naphthaleneacetic acid-induced hyperpolarization of hypocotyl cell membranes in intact seedlings. Planta 196, 706–711 (1995). https://doi.org/10.1007/BF01106764
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DOI: https://doi.org/10.1007/BF01106764