Abstract
Proteins obtained from seedling shoots and floral meristems ofSorghum bicolor (L.) Moench cv. NK 1210 induced mycelial growth in the smut fungus,Sporisorium reilianum in vitro. Proteins precipitated with trichloroacetic acid and ammonium sulfate were equally effective as inducers, although there were minor variations in the pattern of mycelial growth. Hydrolysis of the protein fraction with the proteolytic enzyme pronase E resulted in considerable reduction in the proteins' ability to induce mycelial growth. Digestion of the protein fraction with driselase, resulted in a slight enhancement of biological activity. The results suggest that amino sugar moieties in glycoproteins may act as inducers of mycelial growth inSporisorium reilianum.
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References
Wilson JM, Frederiksen RA. Histopathology of the interaction ofSorghum bicolor andSphacelotheca reiliana. Phytopathol 1970;60:828–32.
Bhaskaran S, Smith RH, Frederiksen RA. A factor that promotes mycelial development inSporisorium reilianum in vitro. Bot Gaz 1991;152:453–59.
Kokontis JM, Ruddat M. Enzymatic hydrolysis of hyphal growth factors forUstilago violacea isolated from the host plantSilene alba. Bot Gaz 1989;150:439–44.
Murashige T, Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 1962;15:473–97.
Fry SC. The Growing Plant Cell Wall: Chemical and metabolic analysis. New York: John Wiley & Sons, 1988: 49–101.
Bradford MM. A rapid and sensitive method for the estimation of microgram quantities of protein utilizing the principle of protein binding. Anal Biochem 1976; 72: 248–54.
Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F. Colorimetric method for determination of sugars and related substances. Anal Chem 1956; 28: 350–56.
Nelson N. A photometric adaptation of the Somogyi method for the determination of glucose. J Biol Chem 1944; 153: 375–80.
Laemmli UK, Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature 1970; 227: 680–85.
Nagai K, Yamaguchi H. Direct demonstration of the essential role of intramolecular high mannose oligosaccharide chains in the folding and assembly of soybean (Glycine max.) lectin polypeptides. J Biochem. 1993; 113: 123–25.
Lu Y, Ye J, Wold F. Isolation of oligomannose type glucans from bean glycoproteins. Anal Biochem 1993; 209: 79–84.
Darvill A, Auger C, Bergmann C, Carlson RW, Cheong J-J, Eberhard S, Hahn M, Lo V-M, Marfa V, Meyer B, Mohnen D, O'Neill MA, Spiro MD, van Halbeek H, York WS, Albersheim P. Oligosaccharins-oligosaccharides that regulate growth, development and defence responses in plants. Glycobiology 1992; 2: 181–98.
Bartnicki-Garcia S. Cell wall chemistry, morphogenesis and taxonomy of fungi. Ann Rev Microbiol 1968; 22: 87–108.
Crawford MS, Kolattukudy PE. Pectate lyase fromFusarium solani f. sp. pisi: purification, characterization, in vitro translation of mRNA and involvement in pathogenicity. Arch Biochem Biophys 1987; 258: 196–205.
Krause DR, Wood CJ, Mclean DJ. Glucoamylase (exo-1,4-α-glucan glucanohydrolase, EC 3.2.1.3) is the major starchdegrading enzyme secreted by the phytopathogenic fungusColletotrichum gloeosporoioides. J Gen Microbiol 1991; 137: 2463–68.
Bhaskaran S, Smith RH. Carbohydrates, invertase activity, growth and dimorphism inSporisorium reilianum. Mycopathologia 1993; 122: 35–41.
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Bhaskaran, S., Smith, R.H. Effect of proteolytic and glycolytic enzymes on a factor inSorghum bicolor that induces mycelial growth in the smut fungus,Sporisorium reilianum . Mycopathologia 130, 95–101 (1995). https://doi.org/10.1007/BF01103457
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DOI: https://doi.org/10.1007/BF01103457