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Analysis of esterase zymograms ofFusarium sambucinum and related species

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Abstract

Esterase zymograms were obtained following polyacrylamide slab gel electrophoresis of protein extractsFusarium sambucinum and related species originating from different geographic locations and different matrices. The sites of esterase activity were recorded, and the Rfs were calculated. The data were used for the construction of phenograms by cluster analysis and nonlinear mapping by computerized classification techniques. The fifteen isolates ofF. sambucinum, the eight isolates ofF. torulosum and the six isolates ofF. spec. nov. each had identical profiles, and are therefore electrophoretically distinct species. The isolates ofF. sarcochroum, one ofF. sambucinum sensu lato (BBA 64280) and fifteen isolates ofF. sambucinum were electrophoretically indistinguishable from each other. We assume they are synonymous. The isolate ofF. bactridioides, one ofF. sambucinum sensu lato (BBA 64993) and eight isolates ofF. torulosum had uniform EST patterns, therefore the two species are electrophoretically identical. We assume they are also synonymous. The remaining three isolates ofF. sambucinum sensu lato are somewhat closely related toF. sambucinum isolates on the basis of our investigations.

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Authors and Affiliations

  1. Plant Protection Institute, Hungarian Academy of Sciences, P.O. Box 102, H-1525, Budapest, Hungary

    Á. Szécsi, Gy. Turóczi & B. Bordás

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  1. Á. Szécsi
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  2. Gy. Turóczi
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  3. B. Bordás
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Szécsi, Á., Turóczi, G. & Bordás, B. Analysis of esterase zymograms ofFusarium sambucinum and related species. Mycopathologia 129, 165–171 (1995). https://doi.org/10.1007/BF01103342

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  • DOI: https://doi.org/10.1007/BF01103342

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