Skip to main content
SpringerLink
Log in

Differentiation ofFusarium sambucinum Fuckel sensu lato and related species by RAPD PCR

  • Published:
Mycopathologia Aims and scope Submit manuscript

Abstract

DNA polymorphisms generated by the random amplified polymorphic DNA polymerase chain reaction (RAPD PCR) were used to analyse 41 isolates investigated in the EuropeanFusarium sambucinum Project (EFSP). Employing ten arbitrary (10-mer) oligonucleotides and simple repeat sequences (M13, (GACA)4) as single primers, informative banding patterns typical for identifying European populations ofFusarium sambucinum Fuckel s. str.,F. torulosum (Berk. & Curt.) Nirenberg andF. venenatum Nirenberg were obtained.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Saiki RK, Scharf S, Faloona F, Mullis KB, Horn GT, Erlich HA, Amheim H. Enzymatic amplification of β-globin genomic sequences and restriction site analysis of diagnosis of sickle cell anemia. Science 1985; 230: 1350–1354.

    Article  CAS  PubMed  Google Scholar 

  2. Mullis KB, Faloona FA. Specific synthesis of DNA in vitro via a polymerase-catalysed chain reaction. Methods Enzymol 1987; 155: 335–350.

    Article  CAS  PubMed  Google Scholar 

  3. Saiki RK, Gelfand DH, Stoffel S, Scharf SJ, Higuchi R, Horn GT, Mullis KB, Ehrlich HA. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 1988; 239: 487–491.

    Article  CAS  PubMed  Google Scholar 

  4. Welsh J, McClelland M. Fingerprinting genomes using PCR with arbitrary primers. Nucl Acids Res 1990; 18: 7213–7218.

    Article  CAS  PubMed  Google Scholar 

  5. Williams JGK, Kubelik AR, Livak J, Rafalski JA, Tingey SV. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucl Acids Res 1990; 18: 6531–6535.

    Article  CAS  PubMed  Google Scholar 

  6. Welsh J, Petersen C, McClelland M. Polymorphisms generated by arbitrarily primed PCR in the mouse: Application to strain identification and genetic mapping. Nucl Acids Res 1991; 19: 303–306.

    Article  CAS  PubMed  Google Scholar 

  7. Caetano-Anollés G, Bassam BJ, Gresshoff PM. DNA amplification fingerprinting using very short arbitrary oligonucleotide primers. Bio/Technol 1991; 9: 553–557.

    Article  Google Scholar 

  8. Zilberstein A, Pnini-Cohen S, Eyal Z, Shuster S, Hillel J, Lavi U. Application of DNA fingerprinting for dctecting genetical variation among isolates of the wheat pathogenMycosphaerella graminicola. In: Chet I, ed. Biotechnology in plant diseases control. Wiley-Liss, 1993: 341–353.

  9. Schönian G, Meusel O, Tietz H-J, Meyer W, Gräser Y, Tausch I, Presber W, Mitchel TG. Identification of clinical strains ofCandida albicans by DNA fingerprinting with the polymerase chain reaction. Mycoses 1993; 36: 171–179.

    Article  PubMed  Google Scholar 

  10. Caetano-Anollés G, Bassam BJ, Gresshoff PM. DNA amplification fingerprinting: A strategy for genome analysis. Plant Mol Biol Rep 1991; 9: 294–307.

    Article  Google Scholar 

  11. Crowhurst RN, Hawthorne BT, Rikkerink EHA, Templeton MD. Differentiation ofFusarium solani f. sp.cucurbitae raccs 1 and 2 by random amplified polymorphic DNA. Curr Genet 1991; 20: 391–396.

    Article  CAS  PubMed  Google Scholar 

  12. Hering O: Rassendifferenzierung beiFusarium oxysporum f. sp.vasinfectum mittels RAPD-PCR (Abstract). Phytomedizin 1993; 23: 51–52.

    Google Scholar 

  13. Hamelin RC, Oulette GB, Bernier L. Identification ofGremmeniella abietina races with random amplified polymorphic DNA Markers. Appl Environ Microbiol 1993; 59: 1752–1755.

    CAS  PubMed  Google Scholar 

  14. Goodwin PH, Annis SL. Rapid identification of genetic variation and pathotype ofLeptosphaeria maculans by random amplified polymorphic DNA assay. Appl Environ Microbiol 1991; 57: 2482–2486.

    CAS  PubMed  Google Scholar 

  15. Schäfer C, Wöstemeyer J. Random primer dependent PCR differentiates aggressive from non-aggressive isolates of the oilseed rape pathogenPhoma lingam (Leptosphaeria maculans). J Phytopathol 1992; 136: 124–136.

    Article  Google Scholar 

  16. Sreenivasaprasad S, Brown AE, Mills PR. DNA sequence variation and interrelationships amongCollectotrichum species causing strawberry anthracnose. Physiol Mol Plant Pathol 1992; 41: 265–281.

    Article  CAS  Google Scholar 

  17. Bayman P, Cotty PJ. Genetic diversity inAspergillus flavus: Association with aflatoxin production and morphology. Can J Bot 1993; 71: 23–31.

    Article  CAS  Google Scholar 

  18. Leung H, Loomis P, Shi Y. Differentiation of the wheat bunt fungi by random amplification of polymorphic DNA (Abstract). Phytopathology 1991; 81: 1190.

    Google Scholar 

  19. Nirenberg HI. Morphological differentiation ofFusarium sambucinum Fuckel s. str.,F. torulosum (Berk & Curt) Nirenberg comb. nov. andF. venenatum Nirenberg sp. nov. Mycopathologia 1995; 129: 131–141 (this issue).

    Article  CAS  PubMed  Google Scholar 

  20. Nirenberg HI. Recent advances in the taxonomy ofFusarium. Stud Mycol 1990; 32: 91–101.

    Google Scholar 

  21. Huey B, Hall J. Hypervariable DNA fingerprinting inEscherichia coli: Minisatellite probes from bacteriophage M13. J Bacteriol 1989; 171: 2528–2532.

    CAS  PubMed  Google Scholar 

  22. Ali S, Müller CR, Epplen JT. DNA fingerprinting by oligonucleotide probes specific for simple repeats. Hum Genet 1986; 74: 239–243.

    Article  CAS  PubMed  Google Scholar 

  23. Weising K, Weigand F, Driesel AJ, Kahl G, Zischler H, Epplen JT. Polymorphic simple GATA/GACA repeats in plant genomes. Nucl Acids Res 1989; 17: 10128.

    Article  CAS  PubMed  Google Scholar 

  24. Sambrook J, Fritsch EF, Maniatis T. Molecular cloning: A laboratory manual, 2. ed. New York: Cold Spring Harbor Laboratory, 1989.

    Google Scholar 

  25. Booth C. The genus Fusarium. Kew: Commonwealth Mycological Institute, 1971: 168, 189.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Microbiology, Federal Biological Research Centre for Agriculture and Forestry, Berlin, Germany

    Olaf Hering & Helgard I. Nirenberg

Authors
  1. Olaf Hering
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Helgard I. Nirenberg
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hering, O., Nirenberg, H.I. Differentiation ofFusarium sambucinum Fuckel sensu lato and related species by RAPD PCR. Mycopathologia 129, 159–164 (1995). https://doi.org/10.1007/BF01103341

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01103341

Key words

Search

Navigation