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Role of external calcium in sodium and chloride transport in the gills of seawater-adaptedMugil capito

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Summary

When the mulletMugil capito is transferred to medium lacking Ca++ (either Ca++-free seawater or distilled water) the passive permeability of the gill to Na+ and Cl− is increased and the activating effect of external K+ on the Na+ and Cl− effluxes in hyposaline media is inhibited. The permeability of the gill increases progressively in proportion to the time of Ca++ deprivation; it declines when Ca++ is added again to the external medium. The active mechanisms for ion excretion are not reversible. At external Ca++ concentrations from 0.1 to 10 mM the Na+ permeability is constant but the activation of Na+ efflux by K+ shows a maximum at a Ca++ concentration of about 1 mM. For activation of Cl− efflux external bicarbonate must be present, in addition to Ca++, suggesting the existence of a Cl−/HCO −3 exchange. The mechanism by which Ca++ controls the passive branchial permeability is thus probably different from that involved in K+ activation of ion excretion. The Ca++ effect on the K+ sensitive ionic excretory mechanisms seems to be related to intracellular Ca++ movements. Thus, on the one hand, substances such as Ruthenium Red and La+++ which both inhibit Ca++ exchange, in media containing Ca++ and HCO −3 also inhibit K+ activation of Na+ and Cl− effluxes; on the other hand, the ionophore A 23187, a stimulator of Ca++ exchange, when added to these media, activates the Na+ and Cl− effluxes; its maximal effect on the Na+ flux occurs at 2 mM Ca++.

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Abbreviations

ASW-Ca :

artificial seawater minus calcium

DW :

deionised water

DWCa :

deionised water with 1 mM Ca++ added

DWCaHCO 3 :

DW with calcium plus bicarbonate

DWHCO 3 :

DW with 1 mM sodium bicarbonate added

FW :

freshwater (tap water)

FWK :

freshwater with K+ added

P. D. :

potential difference

SW :

seawater

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Authors and Affiliations

  1. Groupe de Biologie Marine du Départment de Biologie du Commissariat à l'Energie Atomique, F-06230, Villefranche-sur-Mer, France

    Pierre Pic & Jean Maetz

  2. Station Marine d'Endoume, rue Batterie des Lions, F-13007, Marseille, France

    Pierre Pic & Jean Maetz

Authors
  1. Pierre Pic
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  2. Jean Maetz
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Additional information

The experiments reported in this paper were done with Jean Maetz who tragically died in August 1977. It is the last report about several years of friendly collaboration

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Pic, P., Maetz, J. Role of external calcium in sodium and chloride transport in the gills of seawater-adaptedMugil capito . J Comp Physiol B 141, 511–521 (1981). https://doi.org/10.1007/BF01101474

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