Summary
Horse sera containing anti-tetanus whole IgG molecules, bivalent F(ab')2 fragments and monovalent Fab' fragments were injected in 24 groups of 10–20 mice to compare their protective activity. When tetanus was induced in the mice, either with toxin or with spore suspension ofClostridium tetani 24 or 32 h prior to the injection of the antitoxins, monovalent Fab' was significantly more efficient in conferring protection against tetanus than F(ab')2 or IgG.
Résumé
Des sera de cheval contenant soit des molécules d'IgG entier, des fragments bivalents F(ab')2 ou des fragments monovalents Fab' ont été injectés dans 24 groupes de 10 à 20 souris afin de comparer leur activité protectrice. Lorsque le tétanos a été induit chez les souris, que ce fût avec la toxine ou avec une suspension de spores deClostridium tetani, 24 ou 32 heures avant l'injection des antitoxines, le fragment monovalent Fab' s'est révelé plus efficace de manière significative que le fragment F(ab')2 ou l'IgG dans l'octroi de l'activité protectrice contre le tétanos.
Resumen
Afín de comparar la actividad protectora frente al tétanos de moléculas de IgG enteras, fragmentos F(ab')2 bivalentes y fragmentos Fab' monovalentes, se inyectaron, con suero de caballo que contenía las distintas moleculas, 24 grupos de 10 a 20 ratones. Se indujo el tétanos en los ratones 24 o 32 horas antes de la inoculación con antitoxinas, mediante la toxina o bien con una suspensión de esporas deClostridium tetani. El fragmento monovalente Fab' confirió una protección frente al tétanos significativamente mayor que F(ab')2 y IgG.
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Vaz, C.A.C., Ferreira, H. & Calich, V.L.G. Higher protective activity of Fab' fragment compared to F(ab')2 and IgG on experimental tetanus. Mircen Journal 4, 339–348 (1988). https://doi.org/10.1007/BF01096139
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DOI: https://doi.org/10.1007/BF01096139