Abstract
Brown beans and kidney beans were subjected to two modes of cooking in a household slow cooker: (A), a fixed low setting for 10 h, and (B), a high setting for 2.5 h and a low setting for 7.5 h. Temperature changes in the beans were recorded. With treatment A over 90% of the hemagglutinating and trypsin inhibitor activities occurred after 6 h at which time the temperature had reached 80°C. With treatment B inactivation of these activities was almost complete at the end of 2 h when a maximum temperature of 100°C had been attained. The in vitro digestibility of the bean protein was considerably increased by either treatment. By way of contrast, only 20 min of heating was required to destroy these activities when the beans were brought to a boil in an open vessel.
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References
Bender AE, Reaidi GB (1982) Toxicity of kidney beans (Phaseolus vulgaris) with particular reference to lectins. J Plant Foods 4:15–22
Cartwright GE, Wintrobe MM (1946) Hematologic survey of repatriated American military personnel. J Lab Clin Med 31:886–899
Coffey DG, Uebersax MA, Hosfield GL, Brunner JR (1985) Evaluation of the hemagglutinating activity of low-temperature cooked kidney beans. J. Food Sci 50:78–81, 87
El-Nahry F, Darwich NM, Tharwat S (1977) Effect of preparation and cooking on the value of the local kidney bean (Phaseolus vulgaris var. Giza 3). Qual Plant Plant Foods Hum Nutr 27:141–146
Faschingbauer H, Kofler L (1929) Ueber Giftwirkung von rohen Bohnen und Bhonenkeimlingen. Wiener Klin Wochenschrift 42:1069–1071
Gatfield IL (1980) Loss of dry matter during the industrial preparation of legumes: leaching of trypsin inhibitor from beans during the soaking step. Lebensm — Wiss U — Technol 13:46–48
Grant G, More LJ, McKenzie NH, Pusztai A (1982) The effect of heating on the hemagglutinating activity and nutritional properties of bean (Phaseolus vulgaris) seeds. J Sci Food Agr 33:1324–1326
Griebel C (1950) Erkrankungen durch Bohenflocken (Phaseolus vulgaris L.) und Platterbsen (Lathyrus tingitanus L.). Z Lebensm Untersuch — Forsch 90:191–197
Hoffman M (1975) Crockery Cookery. Bantam Books, New York, NY
Honavar PM, Shih CV, Liener IE (1962) Inhibition of the growth of rats by fractions ofPhaseolus vulgaris. J. Nutr 77:109–114
Hsu HW, Vavak DL, Satterlee LD, Miller GA (1977) A multienzyme technique for estimating protein digestibility. J Food Sci 42:1269–1283
Johnston MB, Caselman MW (1977) Home economics guide. Bull. No. 9/77/8M. University of Missouri, Columbia, MO
Kakade ML, Evans RJ (1965) Nutritive value of navy beans (Phaseolus vulgaris). Brit J Nutr 19:269–276
Kakade ML, Evans RJ (1966) Effect of soaking and germination on the nutritive value of navy beans. J Food Sci 31:781–783
Kakade ML, Hoffa D, Liener IE (1973) Contribution of trypsin inhibitors to the deleterious effects of unheated soybeans fed to rats. J Nutr 103:1772–1778
Kakade ML, Rackis JJ, McGhee JE, Puski G (1974) Determination of trypsin inhibitor activity of soy products: a collaborative analysis of an improved procedure. Cereal Chem 51:376–382
Kobert R (1918) Zur Kenntnis der Lupinen. Chemiker-Ztg 42:245–248
Liener IE (1955) The photometric determination of the hemagglutinating activity of soyin and crude soybean extracts. Arch Biochem Biophys 54:223–231
Liener IE (1980) Toxic Constituents of Plant Foodstuffs. 2nd edn., Academic Press, New York, NY
Liener IE, Thompson R (1980) In vitro and in vivo studies on the digestibility of the major storage protein of the navy bean (Phaseolus vulgaris). Qual Plant Plant Foods Hum Nutr 30:13–25
Noah ND, Bender AE, Reaidi GB, Gilbert RJ (1980) Food poisoning from raw kidney beans. Brit Med J 281–236–237
Pak N, Manteluna A, Araya A (1978) Effect of different types of heat treatment on the hemagglutinin content and protein quality of beans (Phaseolus vulgaris). Arch Latinoamer Nutr 28:184–195
Thompson LU, Rea RZ, Jenkins DJ (1983) Effect of heat processing on hemagglutinin activity in red kidney beans. J Food Sci 48:235–236
Turner RH, Liener IE (1975) The use of glutaraldehyde treated erythrocytes for assaying the agglutinating activity of lectins. Anal Biochem 68:651–653
Uy R, Wold F (1977) 1,4-Butanediol diglycidyl ether coupling of carbohydrates to Sepharose: affinity absorbents for lectins and glycosidases. Anal Biochem 81:98–107
Wolzak A, Bressani R, Brenes RG (1981) A comparison of in vivo and in vitro estimates of protein digestibility of native and thermally processed vegetable protein. Qual Plant Plant Foods Hum Nutr 31:31–43
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Lowgren, M., Liener, I.E. The effect of slow-cooking on the trypsin inhibitor and hemagglutinating activities and in vitro digestibility of brown beans (Phaseolus vulgaris, var.Stella) and kidney beans (Phaseolus vulgaris, var.Montcalm). Plant Food Hum Nutr 36, 147–154 (1986). https://doi.org/10.1007/BF01092141
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DOI: https://doi.org/10.1007/BF01092141