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Inhibition of hepatic microsomal NADPH-cytochrome C (P-450) reductase activity in gari-fed rats

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Abstract

NADPH-cytochrome c (P-450) reductase activities were measured in liver microsomes prepared from male albino rats (110–150 g) fed control and (35% w/w) gari (a dried cassava product) based diets for 8 weeks and sacrificed at intervals of 1, 3, 5, and 8 weeks. After an initial increase (2-fold) in NADPH oxidation in the first week, the gari diet consistently produced marked decreases in theK m andV max values for NADPH oxidation and cytochrome C reduction. Inhibition of cytochrome C reduction was consistently greater than that of NADPH oxidation. Inhibition was generally not dependent on duration of gari feeding. The site of inhibition of electron-transfer was probably between the reduced prosthetic groups (FAD and FMN) and cytochrome C (P-450).

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  1. Department of Biochemistry, University of Nigeria, Nsukka, Nigeria

    Onyechi Obidoa & Gladys U. Uzoka

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  1. Onyechi Obidoa
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  2. Gladys U. Uzoka
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Obidoa, O., Uzoka, G.U. Inhibition of hepatic microsomal NADPH-cytochrome C (P-450) reductase activity in gari-fed rats. Plant Food Hum Nutr 35, 63–72 (1985). https://doi.org/10.1007/BF01092019

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  • DOI: https://doi.org/10.1007/BF01092019

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