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Anti-Hepatitis B surface Antigen monoclonal antibody IgM production in suspension and immobilized cell bioreactors

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Summary

We have cultivated a hybridoma cell-line H505AC in suspension and alginate immobilized cell bioreactors to produce anti-Hepatitis B surface Antigen (anti-HBsAg) monoclonal antibody IgM. The specific IgM production rates correlate linearly with the specific glucose consumption rate in suspension culture with a maximum production rate of 300 μg/106 cells/day. In alginate-cell immobilized airlift bioreactor, a total of 1143 milligrams IgM was produced in 9 days operation with a volumetric productivity 44.1 mg/day.L

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Authors and Affiliations

  1. Development Center for Biotechnology, 81 Chang Hsing Street, Taipei, Taiwan, R.O.C.

    Chi-Wai Kwong, Jih-Han Hsieh, Mei-Jywan Syu & Shan-Shan Chou

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  1. Chi-Wai Kwong
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  2. Jih-Han Hsieh
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  3. Mei-Jywan Syu
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  4. Shan-Shan Chou
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Kwong, CW., Hsieh, JH., Syu, MJ. et al. Anti-Hepatitis B surface Antigen monoclonal antibody IgM production in suspension and immobilized cell bioreactors. Biotechnol Lett 11, 377–382 (1989). https://doi.org/10.1007/BF01089467

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  • DOI: https://doi.org/10.1007/BF01089467

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