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Rat liver 5′-nucleotidase

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Synopsis

Under assay conditions such that there is minimal interference by lysosomal acid phosphatase, the dephosphorylation of nucleoside-5′-monophosphates (AMP or UMP) by rat-liver homogenates at alkaline pH values is attributable to a Mg2+-dependent enzyme (5′-nucleotidase, EC 3.1.3.5). It shows a bimodal distribution between the nuclear and the microsomal fractions, different from that for glucose-6-phosphatase, and also differs in its response to deoxycholate. There is no evidence of an endogenous inhibitor or of enzyme latency. No clear evidence for the existence of more than one 5′-nucleotidase has come from the approaches adopted. These include differential centrifugation, trial of different ions as activators and inhibitors, determination of pH curves, mixed-substrate assays, column chromatography, and the study of liver regeneration (which depresses the activity) and of hepatocarcinogenesis. Results obtained with maleate, Pb2+ ions and Ni2+ ions have a bearing on histochemical and clinical work. The ‘acid phosphatase’ activity of lysosome-rich fractions towards β-glycerophosphate, 5′-AMP, 5′-UMP and 2′-(3′-)UMP, at pH values near 5.0 with no divalent cations added, seems to be due to a single enzyme or group of isoenzymes. The effects of certain anions and cations on this lysosomal activity, and on microsomal 5′-nucleotidase activity, depend on the concentration of the substrate as well as on its nature.

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Author notes

  1. A. A. El-Aaser & E. Reid

    Present address: Wolfson Bioanalytical Centre, University of Surrey Annexe, 14 Falcon Road, S.W.11, London

Authors and Affiliations

  1. Chester Beatty Research Institute (Institute of Cancer Research: Royal Cancer Hospital), S.W.3, London

    A. A. El-Aaser & E. Reid

  2. Bioanalysis and Cancer Laboratory, Department of Biochemistry, University of Surrey, S.W.11, London

    A. A. El-Aaser & E. Reid

Authors
  1. A. A. El-Aaser
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  2. E. Reid
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A. A. El-Aaser is on leave from the Faculty of Medicine, University of Cairo

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El-Aaser, A.A., Reid, E. Rat liver 5′-nucleotidase. Histochem J 1, 417–437 (1969). https://doi.org/10.1007/BF01086983

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  • DOI: https://doi.org/10.1007/BF01086983

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