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Molecular remodelling in hypertrophied hearts from polyomavirus large T-antigen transgenic mice

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Abstract

Polyomavirus large T-antigen transgenic mice develop cardiac hypertrophy characterized by an increase in atrial natriuretic factor and β-myosin heavy chain isoform expression. The aim of this study was to examine changes in proto-oncogene expression in hypertrophied hearts from the transgenic mice. Expression of early growth response-1 (Egr-1) mRNA was detected in hearts from all 15 transgenic mice, but was not detectable in 13 control mice. Reverse transcriptase-polymerase chain reaction experiments usingEgr-1-specific primers confirmed the increase inEgr-1 mRNA in enlarged hearts from the transgenic mice. Expression of c-jun,junD and Ha-ras mRNAs was increased in the transgenic hearts 3, 17 and 2.8-fold, respectively. Western blots showed an increase in c-myc, c-jun and ras protein in hypertrophied transgenic hearts. Immunofluorescence analyses confirmed an increase in Egr-1 and c-jun protein in transgenic cardiomyocytes. Proliferating cell nuclear antigen, Ki-ras and HSP 90 mRNAs were decreased 22, 2.7 and 3-fold, respectively in the transgenic hearts. Not altered in most hypertrophied hearts was expression of c-fos, junB, p53, c-neu, c-myc, HSP70, HSP27, TGF-β or IGF-1 mRNAs. Proto-oncogene and growth factor gene expression in hypertrophy induced by PVLT expression is modulated, with some proto-oncogenes increased and others decreased in expression.

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Author notes

  1. Ala-Eddin Al Moustafa

    Present address: National Research Council, Biotechnology Research Institute, 6100 Royalmount Avenue, H4P 2R2, Montreal, Quebec, Canada

Authors and Affiliations

  1. The Bloomfield Centre for Research in Aging, Lady Davis Institute for Medical Research, Sir Mortimer B. Davis-Jewish General Hospital, 3755 chemin Côte Ste-Catherine, H3T 1E2, Montréal, Québec, Canada

    Emma L. Holder, Ala-Eddin Al Moustafa & Lorraine E. Chalifour

  2. The Department of Microbiology and Immunology, Division of Geriatric Medicine and Aging, McGill Centre for Studies in Aging, McGill University, Montréal, Québec, Canada

    Lorraine E. Chalifour

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  2. Ala-Eddin Al Moustafa
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  3. Lorraine E. Chalifour
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Holder, E.L., Moustafa, AE.A. & Chalifour, L.E. Molecular remodelling in hypertrophied hearts from polyomavirus large T-antigen transgenic mice. Mol Cell Biochem 152, 131–141 (1995). https://doi.org/10.1007/BF01076075

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  • DOI: https://doi.org/10.1007/BF01076075

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