Abstract
The incidence of mucosal hemorrhage induced in rats by the oral administration of taurocholic acid (TCA) and hydrochloric acid (HCl) both singly and in combination was investigated. The effect of prostaglandin 15 (R) 15 methyl-PGE2 methyl ester (Me-PGE2) on the occurrence of hemorrhage induced by TCA with HCl was also studied. The incidence of hemorrhage induced by TCA (10 mM) alone and HCl (160 mM) alone was minimal and not different from that induced by the control solution. The combination of TCA (10 mM) and HCl (160 mM) produced bleeding in 67.7% of animals. The addition of 15 (R) 15 methyl-PGE2 methyl ester (9.9 μM, corresponding to 50 μg/Kg) significantly, reduced the incidence of hemorrhage induced by the combination of TCA with HCl from 67.7% to 31.3%. This suggests that this synthetic prostaglandin may be of value in conditions thought to be associated with reflux of bile into the stomach.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
James AH, Pickering GW: The role of gastric acidity in the pathogenesis of peptic ulcer. Clin Sci Mol Med 8:181–210, 1949
Watkinson G: A study of the changes of pH of gastric contents in peptic ulcer using the 24 hour meal test. Gastroenterology 18:377–390, 1951
Grant R, Grossman MI, Wang KJ, Ivy AC: The cytolytic action of some gastrointestinal secretions and enzymes on the epithelial cells of the gastric and duodenal mucosa. J Cell Physiol 37:137–161, 1951
Du Plessis DJ: Pathogenesis of gastric ulcer. Lancet 1:974–978, 1965
Capper WM, Airth GR, Kilby JO: A test for pyloric regurgitation. Lancet 2:621–623, 1966
Rhodes J, Barnardo DE, Phillips SF, Rovelstad RA, Hofmann AF: Increase reflux of bile into the stomach in patients with gastric ulcer. Gastroenterology 57:241–252, 1969
Black RB, Roberts G, Rhodes J: The effect on healing of bile reflux in gastric ulcer. Gut 12:552–558, 1971
Van Geertrugden: Alterations de la physiologie gastrique sous l'influence de la bile. Leur importance pour la pathogénie de l'ulcère peptique recidivant après gastrectomie. Bull Acad R Med Belg 7:53–117, 1961
Lawson HH: Effect of duodenal contents on the gastric mucosa under experimental conditions. Lancet 1:469–472, 1964
Davenport HW: Destruction of the gastric mucosal barrier by detergents and urea. Gastroenterology 54:175–181, 1968
Geall MG, Phillips SF, Summerskill WHJ: Profile of potential difference in man. Effect of aspirin, alcohol, bile and endogenous acid. Gastroenterology 58:437–443, 1970
Kirk RM: Experimental gastric ulcer in the rat: the separate and combined effects of vagotomy and bile duct implantation into the stomach. Br J Surg 57:521–524, 1970
Black RB, Hole D, Rhodes J: Bile damage to the gastric mucosal barrier: the influence of pH and bile acid concentrations. Gastroenterology 61:178–184, 1971
Saffaie Shirazi S, Den Besten L, Hamza KW: Absorption of bile salts from the gastric mucosal during hemorrhagic shock. Proc Soc Exp Biol Med 140:924–927, 1972
Semple PF, Russell RI: Role of bile acids in the pathogenesis of aspirin-induced gastric mucosal hemorrhage in rats. Gastroenterology 68:67–70, 1975
Cochran KM, MacKenzie JF, Russell RI: Role of taurocholic acid in the production of gastric mucosal damage after ingestion of aspirin. Br Med J 1:183–185, 1975
Rhodes J: Aetiology of gastric ulcer. Gastroenterology 63:171–182, 1972
Robert A, Nezamis JE, Phillips JP: Effect of prostaglandin E1 on gastric secretion and ulcer formation in the rat. Gastroenterology 55:481–487, 1968
Carter DC, Ganesan PA, Bhana D, Karim SMM: The effect of locally administered prostaglandin 15 (R) 15 methyl-E2 methyl ester on gastric ulcer formation in the Shay, rat preparation. Prostaglandins 5:455–463, 1974
Lippmann W: Inhibition of indomethacin-induced gastric ulceration in the rat by perorally-administered synthetic and naturally occurring prostaglandin. Prostaglandins 7:1–10, 1974
Lippmann W: Oral gastric acid secretion—inhibitory activity and antiulcer activity of synthetic prostaglandin analogues: C-15 epimers of 15-hydroxy-15-methyl 1–9 oxoprostanoic acid (AY-22, 469). Prostaglandins 7:223–229, 1974
Usardi MM, Franceshini J, Mandelli V, Daturi S, Mizzotti R: A proposed role for PGE2 in the genesis of stress-induced gastric ulcers. Prostaglandins 8:43–51, 1974
Carmichael HA, Nelson L, Russell RI, Chandra V, Lyon A, Cochran KM: The effect of prostaglandin 15 (R) 15 methyl-E2 methyl ester on aspirin and taurocholic acid-induced gastric mucosal bleeding in rats. Gut 17:33–36, 1976
Robert A, Nezamis JE, Phillips JP: Inhibition of gastric secretion by prostaglandin. Am J Dig Dis 12:1073–1076, 1967
Wilson DE, Phillips C, Levine RA: Inhibition of gastric secretion in man by prostaglandin A1. Gastroenterology 61:201–206, 1971
Carter DC, Ganesan PA, Bhana D, Karim SMM: Inhibition of human gastric secretion by prostaglandin. Br J Surg 60:828–831, 1973
Robert A, Magerlein BJ: 15 methyl PGE2 and 16, 16 dimethyl PGE2: potent inhibitors of gastric secretion. Adv Biosci 9:247–253, 1973
Bedi BS, Debas HT, Gillespie G, Gillespie IE: Effects of bile salts on antral gastrin release. Gastroenterology 60:256–262, 1971
Mann NS: Prevention of bile-induced acute erosive gastritis by prostaglandin E2 and cholestyramine. Gastroenterology 68:946, 1975
Karim SMM, Carter DC, Bhana D, Ganesan PA: Effect of orally and intravenously administered prostaglandin 15 (R) 15 methyl-E2 on gastric secretion in man. Adv Biosc 9:225, 1973
Cohen MM: Prostaglandin E2 prevents gastric mucosal barrier damage. Gastroenterology 68:876, 1975
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Carmichael, H.A., Nelson, L., Russell, R.I. et al. The effect of the synthetic prostaglandin analog 15 (R) 15 methyl-PGE2 methyl ester on gastric mucosal hemorrhage induced in rats by taurocholic acid and hydrochloric acid. Digest Dis Sci 22, 411–414 (1977). https://doi.org/10.1007/BF01071887
Issue Date:
DOI: https://doi.org/10.1007/BF01071887