Abstract
Reanalyses of first-degree biometrical genetic data from previous studies of alcohol preference in the mouse revealed little consistency beyond a basic additive genetic component. A simplified triple-test cross in the rat investigated the genetic architecture of alcohol preference for a 10% (w/v) alcohol solution or water. An initial survey of eight selected and inbred strains identified high- and low-scoring strains, the MNR and the ACI, respectively, which were crossed as tester lines to six strains (the RHA, RLA, TMB, TMD, MNR, and ACI) to produce the required set of largely F1 families. The additive-dominance model proved adequate for males, and directional dominance for low alcohol preference was found on all three measures: alcohol intake, alcohol preference ratio, and alcohol calorie contribution ratio. For females the model was adequate only for alcohol preference ratio, which showed ambidirectional dominance. The relevance of such genetic architecture to an animal model of alcoholism and to the evolution of alcohol drinking in the rat is discussed.
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References
Brewster, D. J. (1968). Genetic analysis of ethanol preference in rats selected for emotional reactivity.J. Hered. 59:283–286.
Broadhurst, P. L. (1960). Applications of biometrical genetics to the inheritance of behaviour. In Eysenck, H. J. (ed.),Experiments in Personality, Vol. 1, Psychogenetics and Psychopharmacology, Routledge and Kegan Paul, London, pp. 1–102.
Broadhurst, P. L. (1978).Drugs and the Inheritance of Behavior: A Survey of Comparative Psychogenetics, Plenum Press, New York.
Broadhurst, P. L. (1979). The availability of behaviorally selected strains of rats.Behav. Genet. 9:323–324.
Broadhurst, P. L., and Jinks, J. L. (1974). What genetical architecture can tell us about the natural selection of behavioural traits. In van Abeelen, J. H. F. (ed.),The Genetics of Behaviour, North-Holland, Amsterdam, pp. 43–63.
Drewek, K. J. (1978). A psychopharmacogenetic investigation of alcohol preference in selected strains of rats, Unpublished Ph.D. thesis, University of Birmingham, Birmingham.
Drewek, K. J. (1980). Inherited drinking and its behavioural correlates. In Eriksson, K., Sinclair, J. D., and Kiianmaa, K. (eds.),Animal Models in Alcohol Research, Academic Press, London, pp. 35–49.
Drewek, K. J., and Broadhurst, P. L. (1979). Alcohol selection by strains of rats selectively bred for behavior.J. Stud. Alc. 40:723–728.
Duncan, D. B. (1953). Multiple range and multipleF tests.Biometrics 11:1–42.
Eriksson, K. (1968). Genetic selection for voluntary alcohol consumption in the albino rat.Science 159:739–741.
Eriksson, K. (1969a). The estimation of heritability for the self-selection of alcohol in the albino rat.Ann. Med. Exp. Biol. Fenn. 47:172–174.
Eriksson, K. (1969b). Factors affecting voluntary alcohol consumption in the albino rat.Ann. Zool. Fenn. 6:227–265.
Eriksson, K. (1971). Inheritance of behaviour towards alcohol in normal and motivated choice situation in mice.Ann. Zool. Fenn. 8:400–405.
Eriksson, K. (1972). Alcohol consumption and blood alcohol in rats selected for their behaviour toward alcohol. In Forsander, O. A., and Eriksson, K. (eds.),International Symposium. Biological Aspects of Alcohol Consumption, Finnish Foundation for Alcohol Studies, Helsinki, pp. 121–125.
Eriksson, K., and Pikkarainen, P. H. (1970). Strain and sex differences in voluntary alcohol consumption, liver ADH activity and aldehyde-oxidising capacity in inbred strains of mice.Jap. J. Stud. Alc. 5:1–7.
Forsander, O. (1978). Alcoholin esiintyminen luonnossa (The occurrence of alcohol in nature).Luonnon Tutkija 82:1–3.
Fulker, D. W. (1972). Applications of a simplified triple-test cross.Behav. Genet. 2:185–198.
Fuller, J. L. (1964). Measurement of alcohol preference in genetic experiments.J. Comp. Physiol. Psychol. 57:85–88.
Fuller, J. L., and Collins, R. L. (1972). Ethanol consumption and preference in mice: A genetic analysis.Ann. N.Y. Acad. Sci. 197:42–48.
Henderson, N. D. (1978). Genetic dominance for low activity in infant mice.J. Comp. Physiol. Psychol. 92:18–125.
Hewitt, J. K., Fulker, D. W., and Broadhurst, P. L. (1981). Genetics of escape-avoidance conditioning in laboratory and wild populations of rats: A biometrical approach.Behav. Genet. 11:533–544.
Jinks, J. L., and Broadhurst, P. L. (1974). How to analyse the inheritance of behaviour in animals—the biometrical approach. In van Abeelen, J. H. F. (ed.),The Genetics of Behaviour, North-Holland, Amsterdam, pp. 1–41.
Jinks, J. L., and Virk, D. S. (1977). A modified triple-test cross analysis to test and allow for inadequate testers.Heredity 39:165–170.
Jinks, J. L., Perkins, J. M., and Breese, E. L. (1969). A general method of detecting additive, dominance and epistatic variation for metrical traits. 11. Application to inbred lines.Heredity 24:45–57.
Kearsey, M. J. (1970). Experimental sizes for detecting dominance variation.Heredity 25:529–542.
Kearsey, M. J., and Jinks, J. L. (1968). A general method for detecting additive, dominance and epistatic variation for metrical traits. 1. Theory.Heredity 23:403–409.
Lester, D., and Freed, E. X. (1973). Criteria for an animal model of alcoholism.Pharmacol. Biochem. Behav. 1:103–107.
Mather, K. (1953). Genetical control of stability in development.Heredity 7:297–336.
Mather, K. (1973).Genetical Structure of Populations, Chapman and Hall, London.
Mather, K., and Jinks, J. L. (1971).Biometrical Genetics: The Study of Continuous Variation, Chapman and Hall, London.
McClearn, G. E., and Rodgers, D. A. (1961). Genetic factors in alcohol preference of laboratory mice.J. Comp. Physiol. Psychol. 54:116–119.
Myers, R. D., and Holman, R. B. (1966). A procedure for eliminating position habit in preference-aversion tests for ethanol and other fluids.Psychonom. Sci. 5:235–236.
Owen, V. A. (1979). The genetic control of shuttle avoidance behaviour in laboratory rats: A triple test-cross investigation, Unpublished doctoral dissertation, University of Birmingham, Birmingham.
Pickett, R. A., and Collins, A. C. (1975). Use of genetic analysis to test the potential role of serotonin in alcohol preference.Life Sci. 17:1291–1296.
Pooni, H. S., and Jinks, J. L. (1976). The efficiency and optimal size of triple-test cross designs for detecting epistatic variation.Heredity 36:215–227.
Thomas, K. (1969). Selection and avoidance of alcohol solutions by two strains of inbred mice and derived generations.Quart. J. Stud. Alc. 30:849–861.
Whitney, G., McClearn, G. E., and DeFries, J. C. (1970). Heritability of alcohol preference in laboratory mice and rats.J. Hered. 61:165–169.
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This work was supported by a grant from the Medical Research Council of Great Britain to Professor J. L. Jinks and the second author and by a postgraduate studentship from the council to the first author.
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Drewek, K.J., Broadhurst, P.L. A simplified triple-test cross analysis of alcohol preference in the rat. Behav Genet 11, 517–531 (1981). https://doi.org/10.1007/BF01070007
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DOI: https://doi.org/10.1007/BF01070007