Abstract
Pseudomonas aeruginosa samples were studied using Mössbauer spectroscopy and electron paramagnetic resonance (EPR). Samples included whole cells, membranes, and soluble fractions from cells which had been grown with57ferric chloride,57ferric citrate or incubated with57ferripyoverdine. These experiments show for the first time thatP. aeruginosa can accumulate iron in a bacterioferritin when grown under conditions of iron limitation and incubated with its cognate ferrisiderophore, ferripyoverdine. Soluble fraction fromP. aeruginosa cells which were grown iron starved and incubated with57ferripyoverdine for 120 min showed the presence of both a ferric and ferrous complex whose Mössbauer spectra matched that of bacterioferritin extracted fromAzotobacter vinelandii and whose EPR spectra showed a characteristic ferritin-like resonance. A second soluble fraction sample from cells which had been grown with57ferric citrate also showed the presence of a species with the same EPR and Mössbauer parameters. In addition Western blotting confirmed the presence of bacterioferritin in the soluble fraction of the cells which had been incubated with ferripyoverdine.
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References
Andrews SC, Findlay JBC, Guest JR, et al. 1991 Physical, chemical and immunological properties of the bacterioferritins ofEscherichia coli, Pseudomonas aeruginosa andAzotobacter vinelandii.Biochim Biophys Acta 1078, 111–116.
Bauminger ER, Harrison PM, Nowik I, Treffry A. 1988 Composition and dynamics of iron in iron-poor ferritin.Hyperf Interact 42, 873–876.
Bauminger ER, Dickson DPE, Cohen SG, Levy A, Ofer S. 1980 Mössbauer spectroscopy ofE. coli and its iron-storage protein.Biophys Biochem Acta 623, 203–212.
Boas JF, Troup GJ. 1971 Electron spin resonance and Mössbauer effect studies of ferritin.Biochim Biophys Acta 229, 68–74.
Harding RA, Royt PW. 1990 Acquisition of iron from citrate byPseudomonas aeruginosa.J. Gen Microbiol 136, 1859–1867.
Kadir FHA, Read NMK, Dickson DDE, et al. 1991 Mössbauer spectroscopic studies of iron inPseudomonas aeruginosa.J. Inorg Biochem 43, 753–758.
Matzanke BF, Muller GI, Bill E, Trautwein AX. 1989 Iron metabolism ofEscherichia coli studied by Mössbauer spectroscopy and biochemical methods.Eur J Biochem 183, 317–379.
Meyer JM, Abdallah MA. 1978 The fluorescent pigment ofPseudomonas fluorescens: biosynthesis, purification and physicochemical properties.J Gen Microbiol 107, 319–328.
Mielczarek EV, Royt PW, Toth-Allen J. 1989 A comparison of citrate, chloride and siderophore transport of iron inPseudomonas aeruginosa. Proc. Conf. on Biomineralization, Durham, NH.
Mielczarek EV, Royt PW, Toth-Allen J. 1990a A study of microbial acquisition of iron using Mössbauer spectroscopy.Hyperf Interact 58, 2365–2372.
Mielczarek EV, Royt PW, Toth-Allen J. 1990b A Mössbauer spectroscopy study of cellular acquisition of iron from pyoverdine byPseudomonas aeruginosa.Biol Metals 3, 34–38.
Moore GR, Mann S, Bannister JV. 1986 Isolation and properties of the complex nonheme-iron-containing cytochromeb 577 (bacterioferritin) fromPseudomonas aeruginosa.J Inorg Biochem 28, 329–336.
Moore GR. 1991 Private communication.
Read NMK, Dickson DPE, Greenwood C. et al. 1990 Evidence from Mössbauer spectroscopy for different forms of iron core inPseudomonas aeruginosa bacterial ferritin.Biochem J 272, 263–264.
St. Pierre TG, Webb J. 1989 Ferritin and hemosiderin: structural and magnetic studies of the iron core. In: Mann S, Webb J, Williams RJP, eds.Biomineradization: Chemical and Biochemical Perspectives. New York: VCH; and references therein.
Watt GD, Frankel RB, Papaefthymiou GC, Spartalian K, Steifel EI. 1986 Redox properties and Mössbauer spectroscopy ofAzotobacter vinelandii bacterioferritin.Biochemistry 25, 4330–4336.
Weir MP, Gibson JF, Peters TJ. 1984 An electron paramagnetic resonance study of ferritin and haemosiderin.Biochem Soc Trans 12, 316–317.
Weir MP, Peters TJ, Gibson JF. 1985 Electron spin resonance studies of splenic ferritin and haemosiderin.Biochem Biophys Acta 828, 298–305.
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Mielczarek, E.V., Andrews, S.C. & Bauminger, R. Mössbauer spectroscopy and electron paramagnetic resonance studies of iron metabolites inPseudomonas aeruginosa: Fe2+ and Fe3+ ferritin in57ferripyoverdine incubated cells and57ferric citrate fed cells. Biometals 5, 87–93 (1992). https://doi.org/10.1007/BF01062219
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DOI: https://doi.org/10.1007/BF01062219