Abstract
Damage of CCC PM2 DNA by 6-hydroxydopamine (6-OHDA) and ascorbic acid (AA), compounds that are both able to release iron from ferritin, was significantly enhanced in the presence of ferritin. H2O2, a product of 6-OHDA autoxidation, did not induce DNA strand breaks in the absence of ferritin and only to a minor extent in the presence of ferritin. DNA damage by 6-OHDA and AA could be reduced by the hydroxyl radical scavenger mannitol, the iron chelator desferrioxamine, and, partly, by a combination of superoxide dismutase and catalase. These inhibitory effects were clearly less pronounced in the presence of ferritin. Ferritin obviously played an important role as a source of iron in the pro-oxidative processes of 6-OHDA and AA. These features might be of importance in cancer therapy since many tumor cells contain elevated ferritin levels.
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Rieth, A.G., Baader, S.L., Lode, H.N. et al. Ferritin enhances production of DNA strand breaks by 6-hydroxydopamine, ascorbic acid and H2O2 in CCC PM2 bacteriophage DNA. Biometals 5, 223–227 (1992). https://doi.org/10.1007/BF01061222
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DOI: https://doi.org/10.1007/BF01061222