Abstract
Smooth muscle cells from the guinea pig urinary bladder were investigated by voltage clamping at the plasma membrane and using an intracellular perfusion technique. Applying adenosine triphosphate (ATP) at a concentration greater than 3 × 10−8 M and at a membrane potential of −100 to −30 mV produced a rise in fast inward transmembrane current. A similar effect was exerted by adenosine diphosphate (ADP) and α-, β-, and β,γ-methylene ATP. Application of guanosine triphosphate, inosine triphosphate, adenosine monophosphate (AMP), and adenosine failed to activate this current. It was found that AMP blocks ATP receptors competitively. No pharmacological differences were found between the latter ATP receptors and those of rat sensory neurons. The ATP receptors were rapidly desensitized and recovered their sensitivity to agonists extremely slowly. Speed of desensitization was reduced by a decrease in ATP concentration.
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Additional information
A. A. Bogomolets Institute of Physiology, Academy of Sciences of the Ukrainian SSR, Kiev. Translated from Neirofiziologiya, Vol. 19, No. 1, pp. 95–100, January–February, 1987.
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Marchenko, S.M., Volkova, T.M. & Fedorov, O.I. Atp-activated ionic permeability in smooth muscle cells isolated from the guinea pig urinary bladder. Neurophysiology 19, 82–86 (1987). https://doi.org/10.1007/BF01056000
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DOI: https://doi.org/10.1007/BF01056000