Skip to main content
SpringerLink
Log in

Rapid characterization of mutagenic aromatic amines in energy-related materials by short column liquid chromatography with electrochemical detection

  • Published:
Archives of Environmental Contamination and Toxicology Aims and scope Submit manuscript

Abstract

A liquid Chromatograph equipped with a short (3 cm) reverse phase column and electrochemical detector was used to characterize aromatic amines in shale oil, synthetic oil, and coal gasification streams. Five major peaks were produced from each sample mixture. The composition of the peaks was determined by high performance liquid chromatography with a long (25 cm) reverse phase column and by gas chromatography/mass spectrometry. Amines in the various peaks included aminonaphthalenes, aminobiphenyls, aminofluorenes, and aminophenanthrenes plus aminoanthracenes. The concentration of aminofluorenes, and aminophenanthrenes plus aminoanthracenes correlated with relative mutagenicity of the base fraction from the oils or tars. The levels of 2- and 3-ringed aromatic amines from shale oil and oil from the Great Plains commercial coal gasification plan were 24 and 184 μg/g tar, respectively, while the respective mutagenicities were 8 and 214 revertants/μg base fraction. This technique has the advantages of high sensitivity and rapid analysis, and could be used to screen for the presence of mutagens in synthetic fuel samples.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Ames BN, McCann J, Yamasaki E (1975) Methods of detecting carcinogens and mutagens with theSalmonella/mammalianmicrosome mutagenicity test. Mutat Res 31:347–364

    PubMed  Google Scholar 

  • Boparai AS, Haugen DA, Suhrbier KM, Schneider JF (1983) An improved procedure for extraction of aromatic bases from synfuel materials. In: Wright CW, Weimer WC, Felix WD (eds) Advanced Techniques in Synthetic Fuels Analysis. US Department of Energy, National Technical Information Center, Springfield, VA, pp 3–11.

    Google Scholar 

  • Conney AH (1982) Induction of microsomal enzymes by foreign chemicals and carcinogenesis by polycyclic aromatic hydrocarbons. Cancer Res 42:4875–4917

    PubMed  Google Scholar 

  • Guerin MR, Ho C-H, Rao TK, Clark BR, Epler JL (1980) Polycyclic aromatic primary amines as determinant chemical mutagens in petroleum substitutes. Environ Res 23:42–53

    PubMed  Google Scholar 

  • Haugen DA, Peak MJ, Suhrbier K, Stamoudis VC (1982) Isolation of mutagenic aromatic amines from a coal conversion oil by cation exchange chromatography. Anal Chem 54:32–37

    Google Scholar 

  • Haugen DA, Stamoudis VC, Peak MJ, Boparai AS (1986) Isolation of a highly mutagenic aminophenanthrene from a coal gasification process tar. Environ Res 39:60–73

    PubMed  Google Scholar 

  • Keleti G, Bern J, Shipiro MA, Gulledge P, Moore GT (1982) Mutagenicity of SRC-11 coal liquefaction waste water treatment residues. Environ Sci Technol 16:826–830

    Google Scholar 

  • Later DW, Lee ML, Wilson BW (1982) Selective detection of amino polycyclic aromatic compounds in solvent refined coal. Anal Chem 54:117–123

    Google Scholar 

  • Later DW, Pelroy RA, Mahlum DD, Wright CW, Lee ML, Weimer WC, Wilson BW (1983) Identification and comparative genotoxicity of polycyclic aromatic hydrocarbons and related nitrogen-containing heteroatomic species in products from coal liquification processes. In: Cooke M, Dennis AJ (eds) Polynuclear Aromatic Hydrocarbons. Batelle Press, Columbus, Ohio, pp 771–783

    Google Scholar 

  • Later DW, Pelroy RA, Stewart DL, McFall T, Booth GM, Lee ML, Tedjamulia M, Castle RN (1984) Microbial mutagenicity of isomeric two-, three-, and four-ring aminopolycyclic aromatic hydrocarbons. Environ Mutagen 6:497–515

    PubMed  Google Scholar 

  • Otagawa T, Stetter JR, Zaromb S (1986) Portable liquid chromatograph for analysis of primary aromatic amines in coalderived materials. J Chromatogr 360:252–259

    Google Scholar 

  • Stamoudis, VC (1986) Chemical and Toxicological Characterization of Morgantown Energy Technology Center Fixed Bed Gasifier Condensates. Argonne National Laboratory Report ANL/SER-6, Argonne, Illinois, 83 pp

  • Tomkins BA, Ho CH (1982) Determination of polycyclic aromatic amines in natural and synthetic crudes. Anal Chem 54:91–96

    Google Scholar 

  • Wilson BW, Pelroy R, Cresto JT (1980) Identification of primary aromatic amines in mutagenically active subfractions from coal liquefaction materials. Mutat Res 79:193–202

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Skidaway Institute of Oceanography, P.O. Box 13687, 31416, Savannah, Georgia

    Richard F. Lee

  2. Environmental Research Division, Argonne National Laboratory, 9700 South Cass Avenue, 60439, Argonne, Illinois

    Kurt C. Pice & Vassilis C. Stamoudis

Authors
  1. Richard F. Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Kurt C. Pice
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Vassilis C. Stamoudis
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lee, R.F., Pice, K.C. & Stamoudis, V.C. Rapid characterization of mutagenic aromatic amines in energy-related materials by short column liquid chromatography with electrochemical detection. Arch. Environ. Contam. Toxicol. 17, 651–656 (1988). https://doi.org/10.1007/BF01055834

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01055834

Keywords

Search

Navigation