Abstract
ApparentK s andV max values, for the transport byThiobacillus A2 of14C-labelled sucrose, hexoses and pentoses, were estimated using flow dialysis and membrane filtration techniques. Transport systems of varying degrees of specificity could be inferred from the data. For most sugars tested including glucose, fructose and arabinose, there was a close correlation between maximum rate of sugar transport and observed growth rate. Differences in transport rate were sufficient to explain slow and fast growth on glucose by wild type and GF strains ofThiobacillus A2.
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Abbreviations
- Butyl PBD:
-
2-(4′-tert-butylphenyl)-5-(4″-biphenylyl)-1,3,4-oxadiazole
- Tris:
-
tris(hydroxymethyl)-amino-methane
- PEP:
-
phosphoenolpyruvate
References
Colowick SP, Womack FC (1969) Binding of diffusible molecules by macromolecules: Rapid measurement by rate of dialysis. J Biol Chem 244: 774–777
Eisenthal R, Cornish-Bowden A (1974) The direct linear plot. A new graphical procedure for estimating enzyme kinetic parameters. Biochem J 139: 715–720
Lineweaver H, Burk D (1934) The determination of enzyme dissociation constants. J Amer Chem Soc 56: 658–666
Matin A, Schleiss M, Perez RC (1980) Regulation of glucose transport and metabolism inThiobacillus novellus. J Bacteriol 142: 639–644
Ramos S, Schuldiner S, Kaback HR (1979). The use of flow dialysis for determinations of 4pH and active transport. In: S Fleischer, L Packer (eds) Methods in enzymology: Biomembranes. Vol 55, Part F. Academic Press, New York, pp 680–688
Saier MH (1977) Bacterial phosphoenolpyruvate: sugar phosphotransferase systems; structural, functional and evolutionary relationships. Bacteriol Rev 41: 856–871
Smith AL, Kelly DP, Wood AP (1980) Metabolism ofThiobacillus A2 grown under autotrophic, mixotrophic and heterotrophic conditions in chemostat culture. J Gen Microbiol 121: 127–138
Taylor BF, Hoare DS (1969) New facultativeThiobacillus and a reevaluation of the heterotrophic potential ofThiobacillus novellus. J Bacteriol 100: 487–497
Wood AP, Kelly DP (1977) Heterotrophic growth ofThiobacillus A2 on sugars and organic acids. Arch Microbiol 113: 265–274
Wood AP, Kelly DP (1978) Triple catabolic pathways for glucose in a fast-growing strain ofThiobacillus A2. Arch Microbiol 117: 309–310
Wood AP, Kelly DP (1979) Glucose catabolism byThiobacillus A2 grown in chemostat culture under carbon or nitrogen limitation. Arch Microbiol 122: 307–312
Wood AP, Kelly DP (1980) Regulation of glucose catabolism inThiobacillus A2 grown in the chemostat under dual limitation by succinate and glucose. Arch Microbiol 128: 91–97
Wood AP, Kelly DP (1981) Mixotrophic growth ofThiobacillus A2 in chemostat culture on formate and glucose. J Gen Microbiol 125: 55–62
Wood AP, Kelly DP (1982) Mechanisms of sugar transport byThiobacillus A2. Arch Microbiol 131: 160–164
Wood AP, Kelly DP, Thurston CF (1977) Simultaneous operation of three catabolic pathways in the metabolism of glucose byThiobacillus A2. Arch Microbiol 113: 265–274
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Wood, A.P., Kelly, D.P. Kinetics of sugar transport byThiobacillus A2. Arch. Microbiol. 131, 156–159 (1982). https://doi.org/10.1007/BF01053998
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DOI: https://doi.org/10.1007/BF01053998