Abstract
Interaction of tetrodotoxin-sensitive proteins of the soluble cytoplasmic fraction of mammalian brain with liposomes was studied. Tetrodotoxin-sensitive proteins were shown to be incorporated in an orderly transmembrane manner into liposomes of a particular chemical composition (phosphatidylcholine-phosphatidylserine-cholesterol, brain phospholipids-cholesterol).
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Literature cited
M. Kates, Techniques of Lipidology, Elsevier (1972).
V. K. Lishko, M. K. Malysheva, and A. P. Stefanov, "Discovery of TTX-sensitive structures in extracts of excitable tissues," Dokl. Akad. Nauk SSSR,248, No. 5, 1257 (1979).
N. I. Mishchenko, M. K. Malysheva, V. A. Zhukareva, and A. F. Skryshevskii, "A study of interaction of liposomes with cytoplasmic brain proteins by the technique of low-angle scattering of x-rays," Dokl. Akad. Nauk SSSR,267, No. 4, 980 (1982).
A. L. Hubbard and Z. A. Cohn, "Specific labels for cell surfaces," in: Biochemical Analysis of Membranes, A. H. Maddy, ed., Chapman and Hall, London (1976), p. 502.
A. D. Bangham, H. W. Hill, and N. G. A. Miller, "Preparation and use of liposomes as model of biological membranes," Methods in Membrane Biology,1, 1 (1974).
G. R. Bartlett, "Phosphorus assay in column chromatography," J. Biol. Chem.,234, No. 2, 466 (1959).
L. A. Cuervo and W. J. Adelman, "Equilibrium and kinetic properties of the interaction between tetrodotoxin and excitable membrane of the squid giant axon," J. Gen. Physiol.,55, No. 2, 309 (1970).
B. Hille, "Pharmacological modifications of the sodium channels of frog nerve," J. Gen. Physiol.,51, No. 1, 199 (1968).
P. W. Holloway, T. C. Markello, and T. L. Leto, "The interaction of cytochrome b5 with lipid vesicles," Biophys. J.,37, No. 1, 63 (1982).
F. Jähnig, "The ordered-fluid transition in lipid bilayers," Mol. Cryst. Liq. Cryst.,63, No. 1, 157 (1981).
H. K. Kimelberg and D. Papahadjopoulos, "Interaction of basic proteins with phospholipid membranes," J. Biol. Chem.,246, No. 5, 1142 (1971).
M. Kunitz, "Crystalline soybean trypsin inhibitor," J. Gen. Physiol.,30, 291 (1947).
U. K. Laemmli, "Cleavage of structural proteins during the assay of the head of bacteriophage T. 4," Nature,227, No. 5, 680 (1970).
S. R. Levinson, J. R. Curatalo, and M. A. Raftery, "A rapid and precise assay for tetrodotoxin binding to detergent extracts of excitable tissues," Anal. Biochem.,99, No. 1, 72 (1979).
O. H. Lowry, N. J. Rosebrough, A. L. Farr, and R. J. Randall, "Protein measurement with Folin phenol reagent," J. Biol. Chem.,193, No. 1, 265 (1951).
M. K. Malysheva, V. K. Lishko, and A. M. Chagovetz, "The association of tetrodotoxin-sensitive, sodium-selective ionophore of brain membranes with liposomes," Biochim. Biophys. Acta,602, No. 1, 70 (1980).
M. K. Malysheva, A. V. Stefanov, A. M. Chagovetz, and V. K. Lishko, "Tetrodotoxin-sensitive proteins in the extracts from excitable membrane," Biochim. Biophys. Acta,688, No. 1, 246 (1982).
J. Matthews, E. X. Albuquerque, and M. E. Eldefrawi, "Influence of batrachotoxin, veratridine, grayanotoxin I and tetrodotoxin on uptake of22Na by rat brain membrane preparations," Life Sci.,25, No. 10, 1651 (1979).
D. D. Sabatini, G. Kreibich, T. Moritomo, and M. Adesnik, "Mechanisms for the incorporation of proteins in membranes and organelles," J. Cell Biol.,92, No. 1, 1 (1982).
H. Sanders, "Preparative isolation of phosphatidylserine from brain," Biochim. Biophys. Acta,144, No. 2, 485 (1967).
J. R. Schwarz, W. Ulbricht, and H. H. Wagner, "The rate of action of tetrodotoxin on myelinated nerve fibres ofXenopus laevis andRana esculenta," J. Physiol. (London),233, No. 1, 167 (1973).
A. Talvenheimo, M. M. Tamkun, and W. A. Catteral, "Reconstitution of neurotoxin-stimulated sodium transport by the voltage-sensitive sodium channel purified from rat brain," J. Biol. Chem.,257. No. 20, 11868 (1982).
J. P. Weigele and R. L. Barchi, "Functional reconstitution of the purified sodium channel protein from rat sarcolemma," Proc. Natl. Acad. Sci. USA,79, No. 11, 3651 (1982).
Additional information
A. A. Bogomolets Institute of Physiology, Academy of Sciences of the Ukrainian SSR, Kiev. A. V. Palladin Institute of Biochemistry, Academy of Sciences of the Ukrainian SSR, Kiev. Translated from Neirofiziologiya, Vol. 16, No. 5, pp. 716–725, September–October, 1984.
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Malysheva, M.K., Lishko, V.K., Zhukareva, V.A. et al. Integration of soluble tetrodotoxin-sensitive brain proteins with liposomal membranes. Neurophysiology 16, 550–558 (1984). https://doi.org/10.1007/BF01052710
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DOI: https://doi.org/10.1007/BF01052710