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Degradation of collagen type IV by C6 astrocytoma cells

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Summary

The key event associated with the initiation of angiogenesis is the localized degradation of the vascular basement membrane. Because of its complex structure, any remodelling and/or modification of the basement membrane must involve the co-ordinated function of a number of different enzyme systems. Type IV collagen is a major protein component (60–90%) of the basement membrane and its degradation is crucial to the initiation of angiogenesis. This study has focused on the mechanisms by which C6 astrocytoma cells degrade human type IV collagen. C6 astrocytoma cells use components of two major degradative pathways to degrade collagen type IV. The major matrix metalloproteinase identified is the activated form (68-KDa) of gelatinase A (72-KDa matrix metalloproteinase) and a serine sensitive 1000-KDa collagenase type IV degrading activity which appears to have the characteristics of a novel extracellular proteasome.

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Authors and Affiliations

  1. Brain Research Laboratories, Experimental Research Unit, Department of Clinical Neurological Sciences, Victoria Hospital Research Institute, University of Western Ontario, London, Ontario, Canada

    Rolando F. Del Maestro, Indrasen S. Vaithilingam & Warren McDonald

Authors
  1. Rolando F. Del Maestro
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  2. Indrasen S. Vaithilingam
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  3. Warren McDonald
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Del Maestro, R.F., Vaithilingam, I.S. & McDonald, W. Degradation of collagen type IV by C6 astrocytoma cells. J Neuro-Oncol 24, 75–81 (1995). https://doi.org/10.1007/BF01052662

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