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Single calcium channels in neurons of rat spinal ganglia

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Abstract

Using a refined patch clamp technique, a study was made of single calcium channels of spinal ganglia neurons on a cell-attached membrane site in newborn rats; these convey the basic (high threshold) component of calcium current. Findings show that currents carried by calcium ions at a concentration of 60 mM in the recording pipet changes from 0.58±0.05 to 0.43±0.05 pA with a change in potential of 20 mV. This corresponds to a channel conductance of 7±0.5 pS. The distribution of open time was monoexponential with a time constant of about 0.75 msec, independent of membrane potential. Distribution of closed time approached a biexponential time course. The fast component (0.8 msec) was voltage-dependent, while the slow component decreased from 22 to 4 msec when depolarization increased by 20 mV. Using experimentally obtained time parameters which describe single calcium channel function, and assuming a three-tier model of the channel, the numerical values of the constants of transition rates between individual states were determined.

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Authors
  1. Ya. M. Shuba
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  2. A. N. Savchenko
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Additional information

A. A. Bogomolets Institute of Physiology, Academy of Sciences of the Ukrainian SSR, Kiev. Translated from Neirofiziologiya, Vol. 17, No. 5, pp. 673–682, September–October, 1985.

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Shuba, Y.M., Savchenko, A.N. Single calcium channels in neurons of rat spinal ganglia. Neurophysiology 17, 490–497 (1985). https://doi.org/10.1007/BF01052187

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  • DOI: https://doi.org/10.1007/BF01052187

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