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A locus controlling the activity of UDP-galactose:GM2(NeuGc) galactosyltransferase in mouse liver is linked to the H-2 complex

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Abstract

Genetic polymorphism in the expression of the GM1(NeuGc) ganglioside has been shown in the liver of inbred strains of mice. Through analysis of the gangliosides of H-2 congenic and recombinant strains, this polymorphism was demonstrated to be controlled by a locus mapped left outside of the H-2 complex on chromosome 17, and the locus was assumed to control the level of the activity of GM1(NeuGc) synthetase, UDP-galactose:GM2(NeuGc) galactosyltransferase (E.C.2.4.1.62) [Hashimotoet al., J Biochem (1983) 94:2049-54].

In the present study we analyzed the genetic linkage between the activity of the galactosyltransferase and the H-2 haplotype. For this purpose, we selected two inbred strains of mice, WHT/Ht and BALB/c, because they have different levels of the transferase activity and show different H-2 haplotypes; the specific activity of the transferase obtained with BALB/c was one-eighth of that with WHT/Ht, and BALB/c expressed the la.7 antigen as one of the products encoded in their H-2d complex, whereas WHT/Ht did not. To analyze the linkage between these two phenotypes, WHT/Ht were mated with BALB/c to obtain the F1 mice, and the female F1 mice were then backcrossed to WHT/Ht. It was found that one half of the backcross generation expressed the la.7 antigen derived from BALB/c and had a significantly lower specific activity of the transferase than that of WHT/Ht, while the other half did not express the la.7 antigen but had the same specific activity of the transferase as that obtained with WHT/Ht.

These results suggest that the locus controlling the level of the transferase activity in mouse liver is linked to the H-2 complex on chromosome 17.

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Abbreviations

NeuGc:

N-glycolylneuraminic acid

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Authors and Affiliations

  1. Metabolism Section, Tokyo Metropolitan Institute of Medical Science, Honkomagome, Bunkyo-ku, 113, Tokyo, Japan

    Yasuhiro Hashimoto, Mikiko Abe, Akemi Suzuki & Tamio Yamakawa

  2. Department of Physiological Chemistry, Tokyo Metropolitan Institute of Medical Science, Honkomagome, Bunkyo-ku, 113, Tokyo, Japan

    Kentaro Iwasaki

Authors
  1. Yasuhiro Hashimoto
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  2. Mikiko Abe
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  3. Akemi Suzuki
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  4. Kentaro Iwasaki
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  5. Tamio Yamakawa
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Additional information

The ganglioside nomenclature is based on the system of Svennerholm, J Neurochem (1963) 10:613-23. The sialic acid species present is shown in parentheses after the ganglioside abbreviation.

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Hashimoto, Y., Abe, M., Suzuki, A. et al. A locus controlling the activity of UDP-galactose:GM2(NeuGc) galactosyltransferase in mouse liver is linked to the H-2 complex. Glycoconjugate J 2, 255–265 (1985). https://doi.org/10.1007/BF01049272

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  • DOI: https://doi.org/10.1007/BF01049272

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