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The structure of a fucose-containingO-glycosidic carbohydrate chain of human platelet glycocalicin

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Abstract

The hydrazinolysis procedure currently used for the release ofN-glycosidic carbohydrate chains was applied to glycocalicin. The resulting mixture of oligosaccharide-alditols was fractionated by high-voltage paper electrophoresis into a neutral (5%) and several acidic fractions. The neutral compounds were passed over Bio-Gel P-4. SomeN-glycosidic oligosaccharide-alditols, of theN-acetyllactosamine type as well as of the oligomannoside type, were found to be present. However, oligosaccharide-alditols derived fromO-glycosidic carbohydrate chains were also found, indicating a partial cleavage of GalNAc1-OSer/Thr linkages under the hydrazinolysis conditions applied. One of the neutralO-glycosidic components was characterized, by 500-MHz1H-NMR spectroscopy in combination with sugar analysis, as the following pentasaccharidealditol:

In addition the afuco analogue of this compound was obtained.

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References

  1. Clemetson KJ, Naim HY, Luscher EF (1981) Proc Natl Acad Sci USA 78:2712–16.

    Article  CAS  Google Scholar 

  2. Solum NO, Hagen I, Slettbakk T (1980) Thromb Res 18:773–85.

    Article  CAS  Google Scholar 

  3. Berndt, MC, Phillips DR (1981) in Research Monographs in Cell and Tissue Physiology, eds. Dingle JT, Gordon JL, Elsevier, Amsterdam, 5:43–75.

    Google Scholar 

  4. Clemetson KJ (1983) Blood Cells 9:319–29.

    CAS  PubMed  Google Scholar 

  5. Korrel SAM, Clemetson KJ, van Halbeek H, Kamerling JP, Sixma, JJ, Vliegenthart JFG (1984) Eur J Biochem 140:571–76.

    Article  CAS  Google Scholar 

  6. Judson PA, Anstee DJ, Clamp JR (1982) Biochem J 205:81–90.

    Article  CAS  Google Scholar 

  7. Tsuji T, Tsunehisa, S, Watanabe Y, Yamamoto K, Tohyama H, Osawa T (1983) J Biol Chem 258:6335–39.

    CAS  PubMed  Google Scholar 

  8. Takasaki S, Mizuochi T, Kobata A (1982) Meth Enzymol 83:263–68.

    Article  CAS  Google Scholar 

  9. Kamerling, JP, Gerwig GJ, Vliegenthart JFG, Clamp JR (1975) Biochem J 151:491–95.

    Article  CAS  Google Scholar 

  10. Kamerling JP, Vliegenthart JFG (1982) Cell Biol Monogr 10:95–125.

    Article  Google Scholar 

  11. Vliegenthart JFG, Dorland L, van Halbeek H (1983) Adv Carbohydr Chem Biochem 41:209–374.

    Article  CAS  Google Scholar 

  12. Mutsaers JHGM, van Halbeek H, Kamerling JP, Vliegenthart JFG (1985) Eur J Biochem 147:568–74.

    Google Scholar 

  13. van Halbeek H, Gerwig GJ, Vliegenthart JFG, Smits HL, van Kerkhof PJM, Kramer MF (1983) Biochim Biophys Acta 747:107–16.

    Article  Google Scholar 

  14. Lamblin G, Boersma A, Lhermitte M, Roussel P, Mutsaers JHGM, van Halbeek H, Vliegenthart JFG (1984) Eur J Biochem 143:227–36.

    Article  CAS  Google Scholar 

  15. van Halbeek H, Dorland L, Vliegenthart JFG, Hull WE, Lamblin G, Lhermitte M, Boersma A, Roussel P (1982) Eur J Biochem 127:7–20.

    Article  Google Scholar 

  16. van Halbeek H, Dorland L, Vliegenthart JFG, Kochetkov NK, Arbatsky NP, Derevitskaya VA (1982) Eur J Biochem 127:21–39.

    Article  Google Scholar 

  17. Dua VK, Dube, VE, Bush CA (1984) Biochim Biophys Acta 802:29–40.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Bio-Organic Chemistry, University of Utrecht, Croesestraat 79, NL-3522 AD, Utrecht, The Netherlands

    Sophia A M Korrel, Herman Van Halbeek, Johannis P Kamerling & Johannes F G Vliegenthart

  2. Theodor Kocher Institute, University of Bern, Freiestrasse 1, Postfach 99, CH-3000, Bern 9, Switzerland

    Kenneth J Clemetson

  3. Department of Haematology, University of Utrecht, Catharijnesingel 101, NL-3511 GV, Utrecht, The Netherlands

    Jan J Sixma

Authors
  1. Sophia A M Korrel
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  2. Kenneth J Clemetson
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  3. Herman Van Halbeek
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  4. Johannis P Kamerling
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  5. Jan J Sixma
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  6. Johannes F G Vliegenthart
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Korrel, S.A.M., Clemetson, K.J., Van Halbeek, H. et al. The structure of a fucose-containingO-glycosidic carbohydrate chain of human platelet glycocalicin. Glycoconjugate J 2, 229–234 (1985). https://doi.org/10.1007/BF01049270

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  • DOI: https://doi.org/10.1007/BF01049270

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