Skip to main content
SpringerLink
Log in

Methanol as a convenient inducer of heat shock element-directed heterologous gene expression in yeast

  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Summary

The addition of sublethal concentrations of methanol to yeast cultures was found to give strong induction of alacZ gene under heat shock element (HSE) control. Methanol addition is therefore an easily-executed alternative to temperature upshift for the induction of HSE-directed gene expression, although in this study it gave slightly lower levels of induction compared to heat shock.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Boorstein, W.R. and Craig, E.A. (1990) Mol. Cell. Biol.10, 3262–3269

    Google Scholar 

  • Finley, D., özkaynak, E. and Varshavsky, A. (1987) Cell48, 1035–1046

    Google Scholar 

  • Guarente, L. and Mason, T. (1983) Cell32, 1279–1286

    Google Scholar 

  • Jacobsen, B.K. and Pelham, H.R.B. (1991) EMBO J.10, 369–375

    Google Scholar 

  • Kirk, N. and Piper, P.W. (1991) Yeast (in press)

  • Leao, C. and van Uden, N. (1982) Biotechnol. Bioeng.24, 1581–1585

    Google Scholar 

  • Leao, C. and van Uden, N. (1985) Appl. Microbiol. Biotechnol.22, 359–363

    Google Scholar 

  • Piper, P.W., Davies, M.W., Curran, B., Lockheart, A., Spalding, A. and Tuite, M.F. (1987) Curr. Genet.11, 595–598

    Google Scholar 

  • Piper, P.W. and Kirk, N. (1991) In.Genetically-Engineered Proteins and Enzymes from Yeasts: Production Control, Wiseman, A. ed. pp387–428 Ellis-Horwood, Chichester, U.K.

    Google Scholar 

  • Sherman, F., Fink, G.R. and Hicks, J.B. (1983) In:Methods in Yeast Genetics pp.62–84 Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.

    Google Scholar 

  • Sorger, P.K. and Pelham, H.R.B. (1987) EMBO J.6, 3035–3041

    Google Scholar 

  • Sorger, P.K. (1991) Cell65, 363–366

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, University College London, WC1E 6BT, London, UK

    Niall Kirk & Peter W. Piper

  2. Department of Chemical and Biochemical Engineering, University College London, WC1E 6BT, London, UK

    Niall Kirk

Authors
  1. Niall Kirk
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Peter W. Piper
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kirk, N., Piper, P.W. Methanol as a convenient inducer of heat shock element-directed heterologous gene expression in yeast. Biotechnol Lett 13, 465–470 (1991). https://doi.org/10.1007/BF01049201

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01049201

Keywords

Search

Navigation