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Binding and precipitating activities ofErythrina lectins with complex type carbohydrates and synthetic cluster glycosides. A comparative study of the lectins fromE. corallodendron, E. cristagalli, E. flabelliformis, andE. indica

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Abstract

Erythrina lectins possess similar structural and carbohydrate binding properties. Recently, tri- and tetra-antennary complex type carbohydrates with non-reducing terminal galactose residues have been shown to be precipitated as tri- and tetravalent ligands, respectively, with certainErythrina lectins [Bhattacharyya L, Haraldsson M, Brewer CF (1988) Biochemistry 27∶1034-41]. The present work describes a comparative study of the binding and precipitating activities of fourErythrina lectins,viz. E. corallodendron, E. cristagalli, E. flabelliformis, andE. indica, with multi-antennary complex type carbohydrates and synthetic cluster glycosides. The results show that though their binding affinities are very similar, theErythrina lectins show large differences in their precipitating activities with the carbohydrates. The results also indicate significant dependence of the precipitating activities of the lectins on the core structure of the carbohydrates. These findings provide a new dimension to the structure-activity relationship of the lectins and their interactions with asparagine-linked carbohydrates.

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Abbreviations

EAL, ECorL, ECL, EFL, and EIL:

represent the lectins from the seeds ofErythrina arborescens,

E. corallodendron, E. cristagalli, E. flabelliformis, andE. indica :

respectively

AFOS:

thetri-antennary complex type oligosaccharide from asialofetuin

AFGP:

the tri-antennary glycopeptide from asialofetuin

MeβGal:

methyl β-d-galactopyranoside

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Authors and Affiliations

  1. Departments of Molecular Pharmacology, and Microbiology and Immunology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, 10461, Bronx, New York, USA

    Lokesh Bhattacharyya & Fred Brewer

  2. Department of Chemistry, Arrhenius Laboratory, University of Stockholm, Sweden

    Martin Haraldsson

  3. Department of Biophysics, The Weizmann Institute of Science, Rehovot, Israel

    Nathan Sharon & Halina Lis

Authors
  1. Lokesh Bhattacharyya
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  2. Martin Haraldsson
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  3. Nathan Sharon
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  4. Halina Lis
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  5. Fred Brewer
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Additional information

Unless stated otherwise all sugars are in thed-configuration.

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Bhattacharyya, L., Haraldsson, M., Sharon, N. et al. Binding and precipitating activities ofErythrina lectins with complex type carbohydrates and synthetic cluster glycosides. A comparative study of the lectins fromE. corallodendron, E. cristagalli, E. flabelliformis, andE. indica . Glycoconjugate J 6, 141–150 (1989). https://doi.org/10.1007/BF01047896

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  • DOI: https://doi.org/10.1007/BF01047896

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