Summary
γ-Glutamyltranspeptidase (γ-GT) was localized in slices of rat lung, at the ultrastructural level, by pre-embedding immunogold labelling. Antiserum was raised against the protein purified from rat kidney. The enzyme was found to be concentrated on the lumenal surface of the non-ciliated (‘Clara’) cells of the bronchiolar epithelium and, to a lesser degree, on the surface of type II alveolar pneumocytes. This immunological localization was consistent with the distribution of reaction product, in both slices and resin sections incubated to demonstrate γ-GT activity. γ-GT is probably involved in the utilization of reduced gluthathione (GSH) present in the fluid lining the airway epithelium.
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Dinsdale, D., Green, J.A., Manson, M.M. et al. The ultrastructural immunolocalization ofγ-glutamyltranspeptidase in rat lung: Correlation with the histochemical demonstration of enzyme activity. Histochem J 24, 144–152 (1992). https://doi.org/10.1007/BF01047464
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DOI: https://doi.org/10.1007/BF01047464