Skip to main content
Log in

Repeated exposure ofEscherichia coli to free chlorine: Production of strains possessing altered sensitivity

  • Published:
Water, Air, and Soil Pollution Aims and scope Submit manuscript

Abstract

E. coli ATCC 11229 was exposed to Cl under demand-free conditions, and surviving colonies examined. Survivors at low degrees of inactivation did not appear to possess altered sensitivity, even when successively exposed and subcultured through 8 passages. Survivors at high degrees of inactivation appeared to have a greatly increased sensitivity towards free Cl. The type culture, and the sensitive strain had identical growth curves, microscopic appearance, and biochemical traits. The initial rate of inactivation at pH 7 appeared to follow saturation-type kinetics, previously reported for organisms and Cl.

Implications for water treatment and reuse are discussed, as well as possible mechanisms explaining the findings of this study.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • American Public Health Association, American Water Works Association, Water Pollution Control Federation: 1975,Standard Methods for the Examination of Water and Wastewater, 14th edition. Washington, D.C.

  • Bates, R. C., Shaffer, P. T. B., and Sutherland, S. M.: 1977,Appl. Environ. Microbiol. 34, 849.

    Google Scholar 

  • Bates, R. C., Sutherland, S. M., and Shaffer, P. T. B.: 1978, In R. L. Jolley, H. Gorchev and D. H. Hamilton, Jr. (ed.),Water Chlorination, Environmental Impact and Health Effects, Volume 2, Ann Arbor Science Publishers, Inc. Ann Arbor, p. 471.

    Google Scholar 

  • Butterfield, C. T., Wattie, E., Megregian, S., and Chambers, C. W.: 1943,Public Health Rep. 61, 157.

    Google Scholar 

  • Camper, A. K. and McFeters, G. A.: 1979,Appl. Environ. Microbiol. 37, 633.

    Google Scholar 

  • Engelbrecht, R. S., Severin, B. F., Masarik, M. T., Farooq, S., Lee, S. H., Haas, C. N., and Lalchandani, A.: 1977,New Microbial Indicators of Disinfection Efficiency. U.S. Environmental Protection Agency, Environmental Protection Technology Series. EPA-600/2-77-052.

  • Engelbrecht, R. S., Weber, M. J., Schmidt, C. A., and Salter, B. L. 1978,Virus Sensitivity to Chlorine Disinfection of Water Supplies. U.S. Environmental Protection Agency, Environmental Protection Technology Series EPA-600/2-78-123.

  • Farkas-Himsley, H.: 1964,Appl. Microbiol. 12, 1.

    Google Scholar 

  • Floyd, F., Sharp, D. G., and Johnson, J. D.: 1979,Environ. Sci. and Tech. 13, 438.

    Google Scholar 

  • Haas, C. N.: 1978, Ph.D. Thesis, University of Illinois at Urbana-Champaign.

  • Haas, C. N.: 1980,Environ. Sci. and Tech. 14, 339.

    Google Scholar 

  • Haas, C. N. and Engelbrecht, R. S.: 1980a,National Conference on Environmental Engineering, American Society of Civil Engineers, New York, p. 646.

    Google Scholar 

  • Haas, C. N. and Engelbrecht, R. S.: 1980b,J. Water Poll. Control Fed. 52, 1976.

    Google Scholar 

  • Rosenkranz, H. S.: 1973,Mutation Research 21, 171.

    Google Scholar 

  • Shih, K. L. and Lederberg, J.: 1976a,Science 192 1141.

    Google Scholar 

  • Shih, K. L. and Lederberg, J.: 1976b,Jour. Bacteriol. 125, 934.

    Google Scholar 

  • Wlodkowski, T. J. and Rosenkranz, H. S.: 1975,Mutation Research 31, 39.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Haas, C.N., Morrison, E.C. Repeated exposure ofEscherichia coli to free chlorine: Production of strains possessing altered sensitivity. Water Air Soil Pollut 16, 233–242 (1981). https://doi.org/10.1007/BF01046857

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01046857

Keywords

Navigation