Abstract
Three bacterial strains, classified as Campylobacter jejuni biotype 1, Campylobacter coli, and NARTG (nalidixic-acid-resistant thermophilic Campylobacters), were tested for survival in water specimens kept at 4, 12, and 20°C. Five different water milieus were compared: sterile physiological saline, chlorinated tap water, dechlorinated tap water, polluted river water, and sterile filtered river water. With few exceptions, all organisms survived better at 4°C than at 12 or 20°C, regardless of the water milieu. Briefest survival was detected at 20°C; no viable Campylobacters could be demonstrated after more than 2 days at this temperature. Of the 5 waiter milieus tested, the highest mean survival time for all strains was obtained with dechlorinated tap water. In this medium, all 3 strains remained viable for 15 days at 4°C, 10 days at 12°C, and 2 days ait 20°C. Briefest survival was obtained in chlorinated tap water. Even residual amounts of Cl2 drastically reduced the survival of all strains tested. Only small variations in viability were detected for 2 of the strains tested after sterile filtration of a water source with a dense bacterial population. The results are discussed in relation to waterborne outbreaks of campylobacteriosis.
Sammendrag
Tre stammer klassifisert som C. jejuni biotype 1, G. coli og NARTG, med henholdsvis serotype LAU 1, LAU 8/11 og LAU 14, ble testet for overlevingsevne i 5 vanntyper ved 4, 12 og 20 °C. Vanntypene var som følger: sterilt fysiologisk saltvann, klorholdig springvann, avklorert springvann, forurenset elvevann og sterilfiltrert elvevann. Lengst overlevelse blant de testede stammene ble funnet for C. jejuni-stammen i fysiologisk saltvann ved 4°C (21 døgn). Den lengste gjennomsnittlig overlevingstid, uansett vanntype og stamme, ble oppnådd ved 4°G (9,9 døgn), fulgt av 12°G (7,5 døgn) og 20°C (1,1 døgn). Som overlevingsmedium viste avklorert drikkevann seg best egnet, uansett stamme og temperatur, men selv små rester av klor (< 0,06 mg Cl2/1) i drikkevannet reduserte overlevingsevnen drastisk. Sterilfiltrering ga noe kortere overlevingsevne for 2 av de 3 testede Campylo-bacter-stammene. Resultatene blir satt i relasjon til vannbårne utbrudd av campylobacteriose.
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Blankenship, L. C. & S. E. Craven: Campylobacter jejuni survival in chicken meat as a function of temperature. Appl. environ. Microbiol. 1982, 44, 88.
Blaser, M. J., H. L. Hardesty, B. Powers & W.-L. L. Wang: Survival of Campylobacter fetus subsp. jejuni in biological milieus. J. clin. Microbiol. 1980, 11, 309–313.
Blaser, M. J., D. N. Taylor & R. A. Feldman: Epidemiology of Campylobacter infections. In: J. P. Butzler (ed.): Campylobacter infection in man and animals. CRC Press, Inc., Roca Raton, Florida 1984, pp. 143–161.
Butzler, J. P. & M. B. Skirrow: Campylobacter enteritis. Clin. Gastroenterol. 1979, 8, 737–765.
Doyle, M. P.: Campylobacter in foods. In J. P. Butzler (ed.): Campylobacter infection in man and animals. CRC Press, Inc., Boca Raton, Florida 1984, pp. 164–180.
Doyle, M. P. & D. J. Roman: Growth and survival of Campylobacter fetus subsp. jejuni as a function of temperature and pH. J. Food Protect. 1981, 44, 596.
Hopkins, R. S., R. Olinsted & G. R. Istre: Endemic Campylobacter jejuni infection in Colorado: Identified risk factors. Amer. J. Public Health. 1984, 47, 244–250.
Kapperud, G. & O. Rosef: Avian wildlife reservoir of Campylobacter fetus subsp. jejuni, Yersinia spp., and Salmonella spp. in Norway. Appl. environ. Microbiol. 1983, 45, 375–380.
Kist, M.: Infektionen durch Campylobacter jejuni/coli. (Campylobacter jejuni/coli infections). Dtsch. med. Wochenschr. 1983, 108, 67–72.
Lauwers, S. & J. L. Penner: Serotyping Campylobacter jejuni and Campylobacter coli on the basis of thermostable antigens. In: J. P. Butzler (ed.): Campylobacter infection in man and animals. CRC Press, Inc., Boca Raton, Florida 1984, pp. 51–59.
Lior, H.: An extended biotyping scheme for Campylobacter jejuni and Campylobacter coli. Meet. Amer. Soc. Microbiol., New Orleans, 1983, p. 358.
Mentzing, L. O.: Waterborne outbreaks of Campylobacter enteritis in central Sweden. Lancet 1981, 11, 352–354.
Palmer, S. R., P. R. Gully & J. M. White: Water-borne outbreak of Campylobacter gastroenteritis. Lancet 1983, 1, 287–290.
Penner, J. L., A. D. Pearson & J. N. Hennessy: Investigation of a waterborne outbreak of Campylobacter jejuni enteritis with a serotyping scheme based on thermostable antigens. J. clin. Microbiol. 1983, 18, 1362–1365.
Prescott, J. F. & D. L. Munroe: Campylobacter jejuni enteritis in man and domestic animals. J. Amer. Vet. Med. Ass. 1982, 181, 1524–1530.
Robinson, D. A.: Infective dose of Campylobacter jejuni in milk. Brit, med. J. 1981, 282, 1584.
Rosef, O.: Isolation of Campylobacter fetus subsp. jejuni from the gallbladder of normal slaughter pigs, using an enrichment procedure. Acta vet. scand. 1981, 22, 1491–1151.
Rosef, O., B. Gondrosen, G. Kapperud & B. Underdal: Isolation and characterization of Campylobacter jejuni and Campylobacter coli from domestic and wild mammals in Norway. Appl. environ. Microbiol. 1983, 46, 855–859.
Rosef, O., B. Gondrosen & G. Kapperud: Campylobacter jejuni and Campylobacter coli as surface contaminants of fresh and frozen poultry carcasses. Int. J. Food Microbiol. 1984, 1, 205–215.
Skirroiv, M. B.: Campylobacter enteritis: a “new” disease. Brit. med. J. 1977, 2, 9–11.
Skirroiv, M. B.: Campylobacter enteritis - the first five years. J. Hyg. 1982, 89, 175–184.
Skirrow, M. B. & J. Benjamin: Differentiation of enteropathogenic Campylobacter. J. clin. Pathol. 1980, 33, 1122.
Svedhem, A., B. Kaijser & E. Sjogren: The occurrence of Campylobacter jejuni in fresh food and survival under different conditions. J. Hyg. 1981, 87, 421–425.
Taylor, D. N., M. Brown & K. T. McDermott: Waterborne transmission of Campylobacter enteritis. Microbial Ecology 1982, 8, 347–354.
Taylor, D. N., K. T. McDermott, J. R. Little, J. G. Wells & M. J. Blaser: Campylobacter enteritis from untreated water in the Rocky Mountains. Ann. intern. Med. 1983, 99, 38–40.
Tiehan, W. & R. L. Vogt: Waterborne Campylobacter gastroenteritis - Vermont. Morbid. Mortal. Wkly. Rep. 1978, 27, 207.
Vogt, R. L., H. E. Sours, T. Barrett, R. A. Feldman, R. J. Dickinson & L. Withered.: Campylobacter enteritis associated with cont- amined water. Ann. intern. Med. 1982, 96, 292–296.
Wang, W.-L., L. B. W. Powers, N. W. Luechtfeld & M. J. Blaser: Effects of disinfectants on Campylobacter jejuni. Appl. environ. Microbiol. 1983, 45, 1202–1205.
Ostensvik, O.: Overlevelse av termostabile koliforme bakterier og fekale streptokokker i vann fra 11 norsike ferskvannsforekomster med forskjellig trofigrad. (Survival at 20°C of fecal coliform bacteria and fecal streptococci in water from 11 Norwegian fresh waters). Norsk Vet. Tidsskr. 1981, 93, 183–187.
Acknowledgments
I thank Georg Kapperud for essential help with various aspects of this survey, and Kari Dommarsnes for excellent technical assistance.
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Gondrosen, B. Survival of Thermotolerant Campylobacters in Water. Acta Vet Scand 27, 1–10 (1986). https://doi.org/10.1186/BF03548554
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DOI: https://doi.org/10.1186/BF03548554