Summary
AnE. coli harboring a vector containing double promoters, a signal sequence, and interferon gene was used. By fitting the feed rate of growth-limiting nutrients to the precalculated demand of the microorganism on the basis of a specific growth rate of 0.1 h−1, fed-batch fermentations were performed. A cell density of 26 g/L was achieved after 46 hrs cultivation at 30°C. The culture was induced by IPTG and produced 1x109 IU/L of human leukocyte interferon.
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References
Cornelis, P., Digneffe, C., and Willemot, K. (1982).Mol. Gen. Genet., 186, 507–511.
Maniatis, T., Fritsch, E. F., and Sambrook, J. (1982). Molecular Cloning, A Laboratory Manual, New York, Cold Spring Harbor Laboratory.
Marston, F. A. O. (1987). The purification of eukaryotic polypeptides experessed inE. coli. In: DNA cloning, D. M. Glover, ed. vol. 3, pp 59–87, IRL Press.
Masui, Y., Coleman, J., and Inouye, M. (1983). Multipurpose expression cloning vehicles inE. coli. In: Experimental manipulation of gene expression, Inouye, M., ed. pp 15–31, Academic Press.
Meyer, H.-P., and Fiechter, A. (1985).Applied Environ. Microbiol. 50, 503–507.
Nelson, M. J. (1944).J. Biol. Chem., 124, 1375.
Wang, H. Y., Cooney, C. L., and Daniel, I. (1979).Biotech. Bioeng., 21, 975–995.
Zabriskie, D. W., and Arcuri, E. J. (1986).Enzyme Microb. Technol., 8, 706–716.
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Jae-Ho, L., Choi, YH., Kang, SK. et al. Production of human leukocyte interferon inEscherichia coli by control of growth rate in fed-batch fermentation. Biotechnol Lett 11, 695–698 (1989). https://doi.org/10.1007/BF01044099
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DOI: https://doi.org/10.1007/BF01044099