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Effects ofStreptomyces hyaluronidase digestion on the histochemical reactions of proteoglycans in cartilage compared with its effects on certain non-cartilaginous tissues

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Summary

To test the value ofStreptomyces hyaluronidase in carbohydrate histochemistry, the effects of digestion with the enzyme on the staining of cartilage and non-cartilaginous tissues by Alcian Blue (AB) pH 1.0, AB pH 2.5, high iron diamine, low iron diamine, aldehyde fuchsin, dialysed iron-ferrocyanide and AB pH 2.5-periodic acid-Schiff were studied by light microscopy. The results obtained lead to the conclusion that theStreptomyces enzyme releases not only hyaluronic acid but also chondroitin sulphates and keratan sulphates in cartilage. Since hyaluronic acid is known to be linked to chondroitin sulphate proteoglycans, the enzyme is of limited value in localizing hyaluronic acid in cartilage. However, it is useful in localizing hyaluronic acid in most non-cartilaginous tissues.

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References

  • Gad, A. &Sylvén, B. (1969) On the nature of the high iron diamine method for sulfomucins.J. Histochem. Cytochem. 17, 156–64.

    Google Scholar 

  • Hardingham, T. E. (1979) The role of link-protein in the structure of cartilage proteoglycan aggregates.Biochem. J. 177, 237–247.

    Google Scholar 

  • Hardingham, T. E., Ewins, R. J. F. &Muir, H. (1976) Cartilage proteoglycans. Structure and heterogeneity of the protein core and the effects of specific protein modifications on the binding to hyaluronate.Biochem. J. 157, 127–43.

    Google Scholar 

  • Hardingham, T. E. &Muir, H. (1972) The specific interaction of hyaluronic acid with cartilage proteoglycans.Biochim. biophys. Acta 279, 401–5.

    Google Scholar 

  • Hardingham, T. E. &Muir, H. (1973) Binding of oligosaccharides of hyaluronic acid to proteoglycans.Biochem. J. 135, 905–8.

    Google Scholar 

  • Hardingham, T. E. &Muir, H. (1974) Hyaluronic acid in cartilage and proteoglycan aggregation.Biochem. J. 139, 565–81.

    Google Scholar 

  • Heinegård, D. &Axelsson, I. (1977) Distribution of keratan sulfate in cartilage proteoglycans.J. biol. Chem. 252, 1971–9.

    Google Scholar 

  • Kageyama, K. (1955) Studies onAspergillus oryzae strains for Sake-brewing. (7) On the proteolytic activities of Rice-koji.J. Ferment. Technol. 33, 28–32.

    Google Scholar 

  • Leppi, T. J. (1968) Morphochemical analysis of mucous cells in the skin and slime glands of hagfishes.Histochemie 15, 68–78.

    Google Scholar 

  • Lev, R. &Spicer, S. S. (1964) Specific staining of sulphate groups with alcian blue at low pH.J. Histochem. Cytochem. 12, 309.

    Google Scholar 

  • Muramatsu, T. (1970) Hyaluronic acid. InPolysaccharide Biochemistry (edited bySuzuki, S., Muramatsu, T. andYamashina, I.), Vol. 1, pp. 403–411. Tokyo: Kyoritsu-Shuppan.

    Google Scholar 

  • Ohya, T. (1971)Streptomyces hyaluronidase (Amano) fromStreptomyces hyalurolyticus nov. sp. InOfficial Note of Amano Pharmaceutical Company, Ltd, Nishiharu, Aichi-ken, Japan, pp. 1–12.

  • Ohya, T. &Kaneko, Y. (1970) Novel hyaluronidase fromStreptomyces.Biochim. biophys. Acta 198, 607–9.

    Google Scholar 

  • Pearse, A. G. E. (1968) Carbohydrates and mucosubstances. InHistochemistry, Theoretical and Applied. Vol. 1, 3rd edn, pp. 294–380. Edinburgh, London, New York: Churchill Livingstone.

    Google Scholar 

  • Poole, A. R., Pidoux, I., Reiner, A., Tang, L.-H., Choi, H. &Rosenberg, L. (1980) Localization of proteoglycan monomer and link protein in the matrix of bovine articular cartilage.J. Histochem. Cytochem. 27, 621–35.

    Google Scholar 

  • Quintarelli, G., Vocaturo, A., Bellocci, M., Rodén, L., Ippolito, E. &Baker, J. R. (1974) Preliminary ultrastructural demonstration of hyaluronic acid-proteoglycan interaction in cartilage matrix (1).Am. J. Anat. 140, 433–8.

    Google Scholar 

  • Sajdera, S. W. &Hascall, V. C. (1969) Protein-polysaccharide complex from bovine nasal cartilage. A comparison of low and high shear extraction procedures.J. biol. Chem. 244, 77–87.

    Google Scholar 

  • Sajdera, S. W., Hascall, V. C., Gregory, J. D. &Dziewiatkowski, D. D. (1970) The proteoglycans of bovine nasal cartilage, structure of the aggregate. InChemistry and Biology of the Intercellular Matrix (edited byBalazs, E. A.), pp. 851–858. New York: Academic Press.

    Google Scholar 

  • Spicer, S. S. (1965) Diamine methods for demonstrating mucosubstances histochemically.J. Histochem. Cytochem. 13, 211–27.

    Google Scholar 

  • Spicer, S. S., Horn, R. G. &Leppi, T. J. (1967) Histochemistry of connective tissue mucopolysaccharides. InThe Connective Tissue (edited byWagner, B. M. andSmith, D. E.), pp. 251–303. Baltimore: Williams & Wilkins.

    Google Scholar 

  • Sugahara, K. (1978) Structure of cartilage proteoglycans.Seikagaku (Biochemistry) 50, 582–7.

    Google Scholar 

  • Thyberg, J., Lohmander, S. &Heinegård, D. (1975) Proteoglycans of hyaline cartilage. Electron microscopic studies on isolated molecules.Biochem. J. 151, 157–66.

    Google Scholar 

  • Weissmann, B., Hadjiioannon, S. &Tornheim, J. (1964) Oligosaccharase activity of β-N-acetyl-d-glucosaminidase of beef liver.J. biol. Chem. 239, 59–63.

    Google Scholar 

  • Yamada, K. (1971) Effects of novel (Streptomyces) hyaluronidase digestion upon some mucosaccharide stainings of the cartilages and aortas in the rabbit and rat.Histochemic 27, 277–89.

    Google Scholar 

  • Yamada, K. &Hirano, K. (1973) The histochemistry of hyaluronic acid-containing mucosubstances.J. Histochem. Cytochem. 21, 469–72.

    Google Scholar 

  • Yamada, K. (1973) The effect of digestion withStreptomyces hyaluronidase upon certain histochemical reactions of hyaluronic acid-containing tissues.J. Histochem. Cytochem. 21, 794–803.

    Google Scholar 

  • Yamada, K. (1974) The effect of digestion with chondroitinases upon certain histochemical reactions of mucosaccharide-containing tissues.J. Histochem. Cytochem. 22, 266–75.

    Google Scholar 

  • Yamada, K. (1980) Effects of digestion withStreptomyces hyaluronidase upon certain histochemical staining reactions of cartilage and other tissues.Proc. 6th Internat. Histochem. Cytochem. Congr., Brighton, p. 406.

  • Yamada, K. &Shimizu, S. (1975) The use ofStreptomyces hyaluronidase in connective tissue specimens for electron microscopic histochemistry of acid mucosaccharides.Connective Tissue 7, 61–71.

    Google Scholar 

  • Yamagata, T., Saito, H., Habuchi, O. &Suzuki, S. (1968) Purification and properties of bacterial chondroitinases and chondrosulfatases.J. biol. Chem. 243, 1523–40.

    Google Scholar 

  • Yamashina, I. (1970) Distribution of mucopolysaccharides in the tissues and organs of animals. InPolysaccharide Biochemistry (edited bySuzuki, S., Muramatsu, T. andYamashina, I.), Vol. 2, pp. 640–659. Tokyo: Kyoritsu-Shuppan Co. Ltd.

    Google Scholar 

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Authors and Affiliations

  1. Department of Anatomy, Nagoya University School of Medicine, Nagoya, Japan

    K. Yamada

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  1. K. Yamada
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Yamada, K. Effects ofStreptomyces hyaluronidase digestion on the histochemical reactions of proteoglycans in cartilage compared with its effects on certain non-cartilaginous tissues. Histochem J 14, 149–160 (1982). https://doi.org/10.1007/BF01041137

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  • DOI: https://doi.org/10.1007/BF01041137

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